A liposome-PCR assay for the ultrasensitive detection of biological toxins

We describe an ultrasensitive immunoassay for detecting biotoxins that uses liposomes with encapsulated DNA reporters, and ganglioside receptors embedded in the bilayer, as a detection reagent. After immobilization of the target biotoxin by a capture antibody and co-binding of the detection reagent,...

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Bibliographic Details
Published in:Nature biotechnology 2006-05, Vol.24 (5), p.555-557
Main Authors: Mason, Jeffrey T, Xu, Lixin, Sheng, Zong-mei, O'Leary, Timothy J
Format: Article
Language:English
Subjects:
Agriculture
Analytical chemistry
Binding sites
Biochemistry
Bioinformatics
Biological and medical sciences
Biomedical and Life Sciences
Biomedical Engineering/Biotechnology
Biomedicine
Biotechnology
Botulinum Toxins - analysis
brief-communication
Cholera Toxin - analysis
Deoxyribonucleic acid
DNA
DNA - chemistry
Fundamental and applied biological sciences. Psychology
Gangliosides - chemistry
Immunoassay - methods
Life Sciences
Lipid Bilayers - chemistry
Liposomes - chemistry
Polymerase Chain Reaction - instrumentation
Polymerase Chain Reaction - methods
Reverse Transcriptase Polymerase Chain Reaction
Sensitivity and Specificity
Toxins
Toxins, Biological - analysis
Waterborne diseases
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Summary:We describe an ultrasensitive immunoassay for detecting biotoxins that uses liposomes with encapsulated DNA reporters, and ganglioside receptors embedded in the bilayer, as a detection reagent. After immobilization of the target biotoxin by a capture antibody and co-binding of the detection reagent, the liposomes are ruptured to release the reporters, which are quantified by real-time PCR. Assays for cholera and botulinum toxins are several orders of magnitude more sensitive than current detection methods.
ISSN:1087-0156
1546-1696
DOI:10.1038/nbt1201