Effects on MC3T3-E1 Cells and In Silico Toxicological Study of Two 6-(Propan-2-yl)-4-methyl-morpholine-2,5-diones

Recently, we found that two cyclodidepsipeptides, 3,6-di-(propan-2-yl)-4-methyl-morpholine-2,5-dione (1) and 3-(2-methylpropyl)-6-(propan-2-yl)-4-methyl-morpholine-2,5-dione (2), are excellent inhibitors of xanthine oxidase. In order to obtain more information about the toxicological potential of co...

Full description

Saved in:
Bibliographic Details
Published in:Natural product communications 2015-08, Vol.10 (8), p.1423-1426
Main Authors: Vukelić-Nikolić, Marija, Kolarević, Ana, Tomović, Katarina, Yancheva, Denitsa, Cherneva, Emiliya, Najman, Stevo, Šmelcerović, Andrija
Format: Article
Language:English
Subjects:
Animals
Cell Line
Cell Proliferation - drug effects
Cell Survival - drug effects
Enzyme Inhibitors - toxicity
Mice
Morpholines - toxicity
Osteoblasts - cytology
Osteoblasts - drug effects
Osteoblasts - enzymology
Xanthine Oxidase - analysis
Citations: Items that this one cites
Online Access:Request full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Recently, we found that two cyclodidepsipeptides, 3,6-di-(propan-2-yl)-4-methyl-morpholine-2,5-dione (1) and 3-(2-methylpropyl)-6-(propan-2-yl)-4-methyl-morpholine-2,5-dione (2), are excellent inhibitors of xanthine oxidase. In order to obtain more information about the toxicological potential of compounds 1 and 2 on bone cells, the current study was designed to evaluate the effect of these compounds on viability and proliferation of MC3T3-E1 cells. Compound 1 showed neither cytotoxic nor stimulatory effect on cell viability, while compound 2 showed a slight stimulatory effect on cell viability. Both studied compounds showed slight stimulatory effects on proliferation of MC3T3-E1 cells, in a dose dependent manner. Additionally, an in silico toxicological study of compounds 1 and 2 was performed, and the results indicate that they have a good probability of safe biological intake.
ISSN:1934-578X
1555-9475
DOI:10.1177/1934578X1501000828