System for evaluation of oxidative stress on in-vitro-produced bovine embryos

Abstract This study proposed a quantitative evaluation of oxidative status (OS) in bovine embryos. Sixteen-cell stage embryos, cultured under 5% O2 , were treated with oxidative stress inducer menadione (0, 1, 2.5 and 5 µmol/l) for 24 h. Blastocyst rate (BLR) was recorded and expanded blastocysts we...

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Bibliographic Details
Published in:Reproductive biomedicine online 2015-10, Vol.31 (4), p.577-580
Main Authors: de Assis, Patricia Monken, Castro, Leticia Signori, Siqueira, Adriano Felipe Perez, Delgado, Juliana de Carvalho, Hamilton, Thais Rose dos Santos, Goissis, Marcelo Demarchi, Mendes, Camilla Mota, Nichi, Marcilio, Visintin, José Antonio, Assumpção, Mayra Elena Ortiz D'Ávila
Format: Article
Language:English
Subjects:
Animals
Blastocyst - cytology
Blastocyst - drug effects
Blastocyst - metabolism
Blastomeres - cytology
Blastomeres - drug effects
Blastomeres - metabolism
Cattle
CellROX®Green
development
Embryo Culture Techniques
embryo oxidative stress
Embryo, Mammalian - cytology
Embryo, Mammalian - drug effects
Embryo, Mammalian - metabolism
Embryonic Development - drug effects
Embryonic Development - physiology
Female
Fertilization in Vitro - veterinary
microscopy of epifluorescence
Microscopy, Fluorescence
Obstetrics and Gynecology
Oxidative Stress - drug effects
Vitamin K 3 - toxicity
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Summary:Abstract This study proposed a quantitative evaluation of oxidative status (OS) in bovine embryos. Sixteen-cell stage embryos, cultured under 5% O2 , were treated with oxidative stress inducer menadione (0, 1, 2.5 and 5 µmol/l) for 24 h. Blastocyst rate (BLR) was recorded and expanded blastocysts were stained with CellROX®Green (CRG; OS evaluation) and evaluated under epifluorescence microscopy (ratio of pixel/blastomere). A significant effect of menadione was observed for BLR ( P = 0.0039), number of blastomeres/embryo ( P < 0.0001) and OS ( P < 0.001). Strong negative correlations were found between BLR and the number of blastomeres with OS evaluation, demonstrating the efficacy of this analysis to evaluate OS levels of IVF bovine embryos.
ISSN:1472-6483
1472-6491
DOI:10.1016/j.rbmo.2015.06.014