Induction of CD8 super(+) T cell-mediated protective immunity against Trypanosoma cruzi

Trypanosoma cruzi was transformed with the Plasmodium yoelii gene encoding the circumsporozoite (CS) protein, which contains the well-characterized CD8 super(+) T cell epitope, SYVPSAEQI. In vivo and in vitro assays indicated that cells infected with the transformed T. cruzi could process and presen...

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Bibliographic Details
Published in:International immunology 1999-02, Vol.11 (2), p.133-141
Main Authors: Miyahira, Y, Kobayashi, S, Takeuchi, T, Kamiyama, T, Nara, T, Nakajima-Shimada, J, Aoki, T
Format: Article
Language:English
Subjects:
Plasmodium yoelii
Trypanosoma cruzi
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Summary:Trypanosoma cruzi was transformed with the Plasmodium yoelii gene encoding the circumsporozoite (CS) protein, which contains the well-characterized CD8 super(+) T cell epitope, SYVPSAEQI. In vivo and in vitro assays indicated that cells infected with the transformed T. cruzi could process and present this malaria parasite-derived class I MHC-restricted epitope. Immunization of mice with recombinant influenza and vaccinia viruses expressing the SYVPSAEQI epitope induced a large number of specific CD8 super(+) T cells that strongly suppressed parasitemia and conferred complete protection against the acute T. cruzi lethal infection. CD8 super(+) T cells mediated this immunity as indicated by the unrelenting parasitemia and high mortality observed in immunized mice treated with anti-CD8 antibody. This study demonstrated, for the first time, that vaccination of mice with vectors designed to induce CD8 super(+) T cells is effective against T. cruzi infection.
ISSN:0953-8178