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Relationships among deoxynivalenol, ergosterol and Fusarium exoantigens in Canadian hard and soft wheat

Soluble extracellular components (exoantigens) from cultures of Fusarium graminearum and F. sporotrichioides were used to produce antisera from chickens for an indirect enzyme immunoassay. This immunoassay was used to estimate Fusarium exoantigen levels in 40 samples of fusarium head blight-infected...

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Bibliographic Details
Published in:International journal of food microbiology 1998-12, Vol.45 (3), p.217-224
Main Authors: Abramson, D, Gan, Z, Clear, R.M, Gilbert, J, Marquardt, R.R
Format: Article
Language:English
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Summary:Soluble extracellular components (exoantigens) from cultures of Fusarium graminearum and F. sporotrichioides were used to produce antisera from chickens for an indirect enzyme immunoassay. This immunoassay was used to estimate Fusarium exoantigen levels in 40 samples of fusarium head blight-infected hard red spring wheat from Manitoba, and in 50 samples of infected soft white winter wheat from Ontario. These wheat samples were also assayed for deoxynivalenol (DON), the predominant Fusarium mycotoxin, and for ergosterol, a metabolite reflecting fungal biomass. Using F. sporotrichioides antisera, the linear correlations between exoantigen level and DON content for the hard and soft wheats had coefficients of 0.80 and 0.76, respectively. With the same antisera, linear correlations between exoantigen level and total ergosterol concentration for the hard and soft wheats had coefficients of 0.66 and 0.81, respectively.
ISSN:0168-1605
1879-3460
DOI:10.1016/S0168-1605(98)00164-0