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Relationships among deoxynivalenol, ergosterol and Fusarium exoantigens in Canadian hard and soft wheat
Soluble extracellular components (exoantigens) from cultures of Fusarium graminearum and F. sporotrichioides were used to produce antisera from chickens for an indirect enzyme immunoassay. This immunoassay was used to estimate Fusarium exoantigen levels in 40 samples of fusarium head blight-infected...
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| Published in: | International journal of food microbiology 1998-12, Vol.45 (3), p.217-224 |
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| Main Authors: | , , , , |
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| container_end_page | 224 |
| container_issue | 3 |
| container_start_page | 217 |
| container_title | International journal of food microbiology |
| container_volume | 45 |
| creator | Abramson, D Gan, Z Clear, R.M Gilbert, J Marquardt, R.R |
| description | Soluble extracellular components (exoantigens) from cultures of
Fusarium graminearum and
F. sporotrichioides were used to produce antisera from chickens for an indirect enzyme immunoassay. This immunoassay was used to estimate
Fusarium exoantigen levels in 40 samples of fusarium head blight-infected hard red spring wheat from Manitoba, and in 50 samples of infected soft white winter wheat from Ontario. These wheat samples were also assayed for deoxynivalenol (DON), the predominant
Fusarium mycotoxin, and for ergosterol, a metabolite reflecting fungal biomass. Using
F. sporotrichioides antisera, the linear correlations between exoantigen level and DON content for the hard and soft wheats had coefficients of 0.80 and 0.76, respectively. With the same antisera, linear correlations between exoantigen level and total ergosterol concentration for the hard and soft wheats had coefficients of 0.66 and 0.81, respectively. |
| doi_str_mv | 10.1016/S0168-1605(98)00164-0 |
| format | article |
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Fusarium graminearum and
F. sporotrichioides were used to produce antisera from chickens for an indirect enzyme immunoassay. This immunoassay was used to estimate
Fusarium exoantigen levels in 40 samples of fusarium head blight-infected hard red spring wheat from Manitoba, and in 50 samples of infected soft white winter wheat from Ontario. These wheat samples were also assayed for deoxynivalenol (DON), the predominant
Fusarium mycotoxin, and for ergosterol, a metabolite reflecting fungal biomass. Using
F. sporotrichioides antisera, the linear correlations between exoantigen level and DON content for the hard and soft wheats had coefficients of 0.80 and 0.76, respectively. With the same antisera, linear correlations between exoantigen level and total ergosterol concentration for the hard and soft wheats had coefficients of 0.66 and 0.81, respectively.</description><identifier>ISSN: 0168-1605</identifier><identifier>EISSN: 1879-3460</identifier><identifier>DOI: 10.1016/S0168-1605(98)00164-0</identifier><identifier>PMID: 9926999</identifier><identifier>CODEN: IJFMDD</identifier><language>eng</language><publisher>Amsterdam: Elsevier B.V</publisher><subject>ANTIGENE ; ANTIGENOS ; ANTIGENS ; Antigens, Fungal - analysis ; Biological and medical sciences ; BLE DUR ; BLE TENDRE ; Canada ; ERGOSTEROL ; Ergosterol - analysis ; Exoantigens ; Fundamental and applied biological sciences. Psychology ; Fungal plant pathogens ; FUSARIUM ; Fusarium - immunology ; Fusarium - isolation & purification ; Fusarium graminearum ; Fusarium species ; Fusarium sporotrichioides ; Generalities. Techniques ; GIBBERELLA ZEAE ; HARD WHEAT ; Immunoenzyme Techniques ; MANITOBA ; ONTARIO ; Phytopathology. Animal pests. Plant and forest protection ; Plant Diseases ; SOFT WHEAT ; Trichothecenes - analysis ; TRIGO DURO ; TRIGO HARINERO ; Triticum - chemistry ; Triticum - microbiology ; TRITICUM AESTIVUM ; VOMITOXIN ; VOMITOXINA ; VOMITOXINE ; Wheat</subject><ispartof>International journal of food microbiology, 1998-12, Vol.45 (3), p.217-224</ispartof><rights>1998 Elsevier Science B.V.</rights><rights>1999 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c442t-ea2cf0ecdb6c8971fe705ceaf0aa016c469fd7be7b64b6befce3b0ad655bc2a43</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,27923,27924</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=1688405$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/9926999$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Abramson, D</creatorcontrib><creatorcontrib>Gan, Z</creatorcontrib><creatorcontrib>Clear, R.M</creatorcontrib><creatorcontrib>Gilbert, J</creatorcontrib><creatorcontrib>Marquardt, R.R</creatorcontrib><title>Relationships among deoxynivalenol, ergosterol and Fusarium exoantigens in Canadian hard and soft wheat</title><title>International journal of food microbiology</title><addtitle>Int J Food Microbiol</addtitle><description>Soluble extracellular components (exoantigens) from cultures of
Fusarium graminearum and
F. sporotrichioides were used to produce antisera from chickens for an indirect enzyme immunoassay. This immunoassay was used to estimate
Fusarium exoantigen levels in 40 samples of fusarium head blight-infected hard red spring wheat from Manitoba, and in 50 samples of infected soft white winter wheat from Ontario. These wheat samples were also assayed for deoxynivalenol (DON), the predominant
Fusarium mycotoxin, and for ergosterol, a metabolite reflecting fungal biomass. Using
F. sporotrichioides antisera, the linear correlations between exoantigen level and DON content for the hard and soft wheats had coefficients of 0.80 and 0.76, respectively. With the same antisera, linear correlations between exoantigen level and total ergosterol concentration for the hard and soft wheats had coefficients of 0.66 and 0.81, respectively.</description><subject>ANTIGENE</subject><subject>ANTIGENOS</subject><subject>ANTIGENS</subject><subject>Antigens, Fungal - analysis</subject><subject>Biological and medical sciences</subject><subject>BLE DUR</subject><subject>BLE TENDRE</subject><subject>Canada</subject><subject>ERGOSTEROL</subject><subject>Ergosterol - analysis</subject><subject>Exoantigens</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Fungal plant pathogens</subject><subject>FUSARIUM</subject><subject>Fusarium - immunology</subject><subject>Fusarium - isolation & purification</subject><subject>Fusarium graminearum</subject><subject>Fusarium species</subject><subject>Fusarium sporotrichioides</subject><subject>Generalities. Techniques</subject><subject>GIBBERELLA ZEAE</subject><subject>HARD WHEAT</subject><subject>Immunoenzyme Techniques</subject><subject>MANITOBA</subject><subject>ONTARIO</subject><subject>Phytopathology. Animal pests. Plant and forest protection</subject><subject>Plant Diseases</subject><subject>SOFT WHEAT</subject><subject>Trichothecenes - analysis</subject><subject>TRIGO DURO</subject><subject>TRIGO HARINERO</subject><subject>Triticum - chemistry</subject><subject>Triticum - microbiology</subject><subject>TRITICUM AESTIVUM</subject><subject>VOMITOXIN</subject><subject>VOMITOXINA</subject><subject>VOMITOXINE</subject><subject>Wheat</subject><issn>0168-1605</issn><issn>1879-3460</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1998</creationdate><recordtype>article</recordtype><recordid>eNqFkV1rFDEUhoModa3-hEIuRBQcTeYjM7kSWawVlgp-XIczyclsZCZZk5na_nvT3WV72Zscwvue9yTPIeSCsw-ccfHxZz66ggvWvJXdO5ZvdcGekBXvWllUtWBPyepkeU5epPSHMdZUFTsjZ1KWQkq5IsMPHGF2waet2yUKU_ADNRhu77y7gRF9GN9TjENIM8YwUvCGXi4JolsmircB_OwG9Ik6T9fgwTjwdAvR7J0p2Jn-2yLML8kzC2PCV8d6Tn5ffvm1vio2379-W3_eFLquy7lAKLVlqE0vdCdbbrFljUawDCB_RtdCWtP22Pai7kWPVmPVMzCiaXpdQl2dkzeH3F0MfxdMs5pc0jiO4DEsSfG2ZA1nbTY2B6OOIaWIVu2imyDeKc7UPWC1B6zu6SnZqT1gxXLfxXHA0k9oTl1Holl_fdQhaRhtBK9deggXXVfnLZxiLAQFQ8yW6w3PAXlOW4msfzromGHdOIwqaYdeo3ER9axMcI889D9aQKNX</recordid><startdate>19981222</startdate><enddate>19981222</enddate><creator>Abramson, D</creator><creator>Gan, Z</creator><creator>Clear, R.M</creator><creator>Gilbert, J</creator><creator>Marquardt, R.R</creator><general>Elsevier B.V</general><general>Elsevier</general><scope>FBQ</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7T7</scope><scope>8FD</scope><scope>C1K</scope><scope>FR3</scope><scope>M7N</scope><scope>P64</scope></search><sort><creationdate>19981222</creationdate><title>Relationships among deoxynivalenol, ergosterol and Fusarium exoantigens in Canadian hard and soft wheat</title><author>Abramson, D ; Gan, Z ; Clear, R.M ; Gilbert, J ; Marquardt, R.R</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c442t-ea2cf0ecdb6c8971fe705ceaf0aa016c469fd7be7b64b6befce3b0ad655bc2a43</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1998</creationdate><topic>ANTIGENE</topic><topic>ANTIGENOS</topic><topic>ANTIGENS</topic><topic>Antigens, Fungal - analysis</topic><topic>Biological and medical sciences</topic><topic>BLE DUR</topic><topic>BLE TENDRE</topic><topic>Canada</topic><topic>ERGOSTEROL</topic><topic>Ergosterol - analysis</topic><topic>Exoantigens</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Fungal plant pathogens</topic><topic>FUSARIUM</topic><topic>Fusarium - immunology</topic><topic>Fusarium - isolation & purification</topic><topic>Fusarium graminearum</topic><topic>Fusarium species</topic><topic>Fusarium sporotrichioides</topic><topic>Generalities. Techniques</topic><topic>GIBBERELLA ZEAE</topic><topic>HARD WHEAT</topic><topic>Immunoenzyme Techniques</topic><topic>MANITOBA</topic><topic>ONTARIO</topic><topic>Phytopathology. Animal pests. Plant and forest protection</topic><topic>Plant Diseases</topic><topic>SOFT WHEAT</topic><topic>Trichothecenes - analysis</topic><topic>TRIGO DURO</topic><topic>TRIGO HARINERO</topic><topic>Triticum - chemistry</topic><topic>Triticum - microbiology</topic><topic>TRITICUM AESTIVUM</topic><topic>VOMITOXIN</topic><topic>VOMITOXINA</topic><topic>VOMITOXINE</topic><topic>Wheat</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Abramson, D</creatorcontrib><creatorcontrib>Gan, Z</creatorcontrib><creatorcontrib>Clear, R.M</creatorcontrib><creatorcontrib>Gilbert, J</creatorcontrib><creatorcontrib>Marquardt, R.R</creatorcontrib><collection>AGRIS</collection><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Industrial and Applied Microbiology Abstracts (Microbiology A)</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>Engineering Research Database</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Biotechnology and BioEngineering Abstracts</collection><jtitle>International journal of food microbiology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Abramson, D</au><au>Gan, Z</au><au>Clear, R.M</au><au>Gilbert, J</au><au>Marquardt, R.R</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Relationships among deoxynivalenol, ergosterol and Fusarium exoantigens in Canadian hard and soft wheat</atitle><jtitle>International journal of food microbiology</jtitle><addtitle>Int J Food Microbiol</addtitle><date>1998-12-22</date><risdate>1998</risdate><volume>45</volume><issue>3</issue><spage>217</spage><epage>224</epage><pages>217-224</pages><issn>0168-1605</issn><eissn>1879-3460</eissn><coden>IJFMDD</coden><abstract>Soluble extracellular components (exoantigens) from cultures of
Fusarium graminearum and
F. sporotrichioides were used to produce antisera from chickens for an indirect enzyme immunoassay. This immunoassay was used to estimate
Fusarium exoantigen levels in 40 samples of fusarium head blight-infected hard red spring wheat from Manitoba, and in 50 samples of infected soft white winter wheat from Ontario. These wheat samples were also assayed for deoxynivalenol (DON), the predominant
Fusarium mycotoxin, and for ergosterol, a metabolite reflecting fungal biomass. Using
F. sporotrichioides antisera, the linear correlations between exoantigen level and DON content for the hard and soft wheats had coefficients of 0.80 and 0.76, respectively. With the same antisera, linear correlations between exoantigen level and total ergosterol concentration for the hard and soft wheats had coefficients of 0.66 and 0.81, respectively.</abstract><cop>Amsterdam</cop><pub>Elsevier B.V</pub><pmid>9926999</pmid><doi>10.1016/S0168-1605(98)00164-0</doi><tpages>8</tpages></addata></record> |
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| ispartof | International journal of food microbiology, 1998-12, Vol.45 (3), p.217-224 |
| issn | 0168-1605 1879-3460 |
| language | eng |
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| source | ScienceDirect Freedom Collection |
| subjects | ANTIGENE ANTIGENOS ANTIGENS Antigens, Fungal - analysis Biological and medical sciences BLE DUR BLE TENDRE Canada ERGOSTEROL Ergosterol - analysis Exoantigens Fundamental and applied biological sciences. Psychology Fungal plant pathogens FUSARIUM Fusarium - immunology Fusarium - isolation & purification Fusarium graminearum Fusarium species Fusarium sporotrichioides Generalities. Techniques GIBBERELLA ZEAE HARD WHEAT Immunoenzyme Techniques MANITOBA ONTARIO Phytopathology. Animal pests. Plant and forest protection Plant Diseases SOFT WHEAT Trichothecenes - analysis TRIGO DURO TRIGO HARINERO Triticum - chemistry Triticum - microbiology TRITICUM AESTIVUM VOMITOXIN VOMITOXINA VOMITOXINE Wheat |
| title | Relationships among deoxynivalenol, ergosterol and Fusarium exoantigens in Canadian hard and soft wheat |
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