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Iodine disinfection of a model bacteriophage, MS2, demonstrating apparent rebound
MS2 coliphage viruses suspended in buffered distilled water were rapidly inactivated by
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| Published in: | Water research (Oxford) 1999, Vol.33 (1), p.169-179 |
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| Main Authors: | , |
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| Language: | English |
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| cited_by | cdi_FETCH-LOGICAL-c464t-24fe5f4737e9345d8e34d85f1fe2b471a8b38bb078fccde3e3f582e419c4b13 |
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| cites | cdi_FETCH-LOGICAL-c464t-24fe5f4737e9345d8e34d85f1fe2b471a8b38bb078fccde3e3f582e419c4b13 |
| container_end_page | 179 |
| container_issue | 1 |
| container_start_page | 169 |
| container_title | Water research (Oxford) |
| container_volume | 33 |
| creator | Brion, Gail M. Silverstein, JoAnn |
| description | MS2 coliphage viruses suspended in buffered distilled water were rapidly inactivated by |
| doi_str_mv | 10.1016/S0043-1354(98)00189-4 |
| format | article |
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mg/L iodine doses, losing 6
logs (99.9999%) of infectivity within less than 3
min contact time. The effect of pH on MS2 inactivation within the range of 6 to 8 was not statistically significant. However, in the presence of dissolved organic substances, such as detergents and proteins, the inactivation of MS2 viruses decreased significantly to less than 4
logs (99.99%). Of special interest was that in the presence of beef extract proteins, an apparent reversal of MS2 inactivation, dubbed rebound, was observed. It was observed that after an initial 5 to 6
log reduction in infectivity, a consistent and statistically significant increase in the number of plaque forming units (PFU), as much as 2
logs, was measured. MS2 rebound occurred only when the oxidized iodine residual had been quickly consumed by beef extract proteins in solution. Neither virus particle aggregation nor water salinity were found to account for the increase in PFU values. Based on other investigators' suggestions that iodine disinfection caused changes to viral protein coats, it was hypothesized that conformational changes in MS2's protein coat caused by iodine would result in a change in the isoelectric focusing point of whole MS2 virions. A shift in isoelectric focusing point from an acidic pH value of 3.9 to more basic values, and a dispersion of the virus band after exposure to high levels of iodine was observed, supporting the hypothesis that iodine caused changes in the charge distribution characteristics of the protein coat.</description><identifier>ISSN: 0043-1354</identifier><identifier>EISSN: 1879-2448</identifier><identifier>DOI: 10.1016/S0043-1354(98)00189-4</identifier><identifier>CODEN: WATRAG</identifier><language>eng</language><publisher>Oxford: Elsevier Ltd</publisher><subject>Action of physical and chemical agents ; Applied sciences ; Biological and medical sciences ; Drinking water and swimming-pool water. Desalination ; Exact sciences and technology ; Fundamental and applied biological sciences. Psychology ; iodine disinfection ; M52 ; Microbiology ; Pollution ; Virology ; viruses ; Water treatment and pollution</subject><ispartof>Water research (Oxford), 1999, Vol.33 (1), p.169-179</ispartof><rights>1998</rights><rights>1999 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c464t-24fe5f4737e9345d8e34d85f1fe2b471a8b38bb078fccde3e3f582e419c4b13</citedby><cites>FETCH-LOGICAL-c464t-24fe5f4737e9345d8e34d85f1fe2b471a8b38bb078fccde3e3f582e419c4b13</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,4024,27923,27924,27925</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=1631647$$DView record in Pascal Francis$$Hfree_for_read</backlink></links><search><creatorcontrib>Brion, Gail M.</creatorcontrib><creatorcontrib>Silverstein, JoAnn</creatorcontrib><title>Iodine disinfection of a model bacteriophage, MS2, demonstrating apparent rebound</title><title>Water research (Oxford)</title><description>MS2 coliphage viruses suspended in buffered distilled water were rapidly inactivated by <5
mg/L iodine doses, losing 6
logs (99.9999%) of infectivity within less than 3
min contact time. The effect of pH on MS2 inactivation within the range of 6 to 8 was not statistically significant. However, in the presence of dissolved organic substances, such as detergents and proteins, the inactivation of MS2 viruses decreased significantly to less than 4
logs (99.99%). Of special interest was that in the presence of beef extract proteins, an apparent reversal of MS2 inactivation, dubbed rebound, was observed. It was observed that after an initial 5 to 6
log reduction in infectivity, a consistent and statistically significant increase in the number of plaque forming units (PFU), as much as 2
logs, was measured. MS2 rebound occurred only when the oxidized iodine residual had been quickly consumed by beef extract proteins in solution. Neither virus particle aggregation nor water salinity were found to account for the increase in PFU values. Based on other investigators' suggestions that iodine disinfection caused changes to viral protein coats, it was hypothesized that conformational changes in MS2's protein coat caused by iodine would result in a change in the isoelectric focusing point of whole MS2 virions. A shift in isoelectric focusing point from an acidic pH value of 3.9 to more basic values, and a dispersion of the virus band after exposure to high levels of iodine was observed, supporting the hypothesis that iodine caused changes in the charge distribution characteristics of the protein coat.</description><subject>Action of physical and chemical agents</subject><subject>Applied sciences</subject><subject>Biological and medical sciences</subject><subject>Drinking water and swimming-pool water. Desalination</subject><subject>Exact sciences and technology</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>iodine disinfection</subject><subject>M52</subject><subject>Microbiology</subject><subject>Pollution</subject><subject>Virology</subject><subject>viruses</subject><subject>Water treatment and pollution</subject><issn>0043-1354</issn><issn>1879-2448</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1999</creationdate><recordtype>article</recordtype><recordid>eNqFkE1LAzEQhoMoWKs_QchBRKGrySa7mz2JFD8KFZF6D9lkUiPbZE22gv_ebSt69DSX55135kHolJIrSmh5vSCEs4yygl_U4pIQKuqM76ERFVWd5ZyLfTT6RQ7RUUrvhJA8Z_UIvcyCcR6wccl5C7p3weNgscKrYKDFjdI9RBe6N7WECX5a5BNsYBV86qPqnV9i1XUqgu9xhCasvTlGB1a1CU5-5hgt7u9ep4_Z_PlhNr2dZ5qXvB_uslBYXrEKasYLI4BxIwpLLeQNr6gSDRNNQyphtTbAgNlC5MBprXlD2Rid77Z2MXysIfVy5ZKGtlUewjpJWuWkzkX5P8hKSivGBrDYgTqGlCJY2UW3UvFLUiI3nuXWs9xIlLWQW8-SD7mznwKVtGptVF679BcuGS2HN8foZofB4OTTQZRJO_AajIuDdmmC-6foGxnXkYg</recordid><startdate>1999</startdate><enddate>1999</enddate><creator>Brion, Gail M.</creator><creator>Silverstein, JoAnn</creator><general>Elsevier Ltd</general><general>Elsevier Science</general><scope>IQODW</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QH</scope><scope>7UA</scope><scope>C1K</scope></search><sort><creationdate>1999</creationdate><title>Iodine disinfection of a model bacteriophage, MS2, demonstrating apparent rebound</title><author>Brion, Gail M. ; Silverstein, JoAnn</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c464t-24fe5f4737e9345d8e34d85f1fe2b471a8b38bb078fccde3e3f582e419c4b13</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1999</creationdate><topic>Action of physical and chemical agents</topic><topic>Applied sciences</topic><topic>Biological and medical sciences</topic><topic>Drinking water and swimming-pool water. Desalination</topic><topic>Exact sciences and technology</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>iodine disinfection</topic><topic>M52</topic><topic>Microbiology</topic><topic>Pollution</topic><topic>Virology</topic><topic>viruses</topic><topic>Water treatment and pollution</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Brion, Gail M.</creatorcontrib><creatorcontrib>Silverstein, JoAnn</creatorcontrib><collection>Pascal-Francis</collection><collection>CrossRef</collection><collection>Aqualine</collection><collection>Water Resources Abstracts</collection><collection>Environmental Sciences and Pollution Management</collection><jtitle>Water research (Oxford)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Brion, Gail M.</au><au>Silverstein, JoAnn</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Iodine disinfection of a model bacteriophage, MS2, demonstrating apparent rebound</atitle><jtitle>Water research (Oxford)</jtitle><date>1999</date><risdate>1999</risdate><volume>33</volume><issue>1</issue><spage>169</spage><epage>179</epage><pages>169-179</pages><issn>0043-1354</issn><eissn>1879-2448</eissn><coden>WATRAG</coden><abstract>MS2 coliphage viruses suspended in buffered distilled water were rapidly inactivated by <5
mg/L iodine doses, losing 6
logs (99.9999%) of infectivity within less than 3
min contact time. The effect of pH on MS2 inactivation within the range of 6 to 8 was not statistically significant. However, in the presence of dissolved organic substances, such as detergents and proteins, the inactivation of MS2 viruses decreased significantly to less than 4
logs (99.99%). Of special interest was that in the presence of beef extract proteins, an apparent reversal of MS2 inactivation, dubbed rebound, was observed. It was observed that after an initial 5 to 6
log reduction in infectivity, a consistent and statistically significant increase in the number of plaque forming units (PFU), as much as 2
logs, was measured. MS2 rebound occurred only when the oxidized iodine residual had been quickly consumed by beef extract proteins in solution. Neither virus particle aggregation nor water salinity were found to account for the increase in PFU values. Based on other investigators' suggestions that iodine disinfection caused changes to viral protein coats, it was hypothesized that conformational changes in MS2's protein coat caused by iodine would result in a change in the isoelectric focusing point of whole MS2 virions. A shift in isoelectric focusing point from an acidic pH value of 3.9 to more basic values, and a dispersion of the virus band after exposure to high levels of iodine was observed, supporting the hypothesis that iodine caused changes in the charge distribution characteristics of the protein coat.</abstract><cop>Oxford</cop><pub>Elsevier Ltd</pub><doi>10.1016/S0043-1354(98)00189-4</doi><tpages>11</tpages></addata></record> |
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| identifier | ISSN: 0043-1354 |
| ispartof | Water research (Oxford), 1999, Vol.33 (1), p.169-179 |
| issn | 0043-1354 1879-2448 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_17209286 |
| source | ScienceDirect Journals |
| subjects | Action of physical and chemical agents Applied sciences Biological and medical sciences Drinking water and swimming-pool water. Desalination Exact sciences and technology Fundamental and applied biological sciences. Psychology iodine disinfection M52 Microbiology Pollution Virology viruses Water treatment and pollution |
| title | Iodine disinfection of a model bacteriophage, MS2, demonstrating apparent rebound |
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