Mapping of ER gene CpG island methylation by methylation-specific polymerase chain reaction

Southern analysis has shown that DNA from 25% of primary estrogen receptor (ER) alpha -negative breast tumors displays aberrant methylation at one site within the ER gene CpG island. To examine more sites and increase sensitivity, we developed a methylation-specific PCR assay to map methylation of t...

Full description

Saved in:
Bibliographic Details
Published in:Cancer research (Chicago, Ill.) Ill.), 1998-06, Vol.58 (12), p.2515-2519
Main Authors: LAPIDUS, R. G, NASS, S. J, BUTASH, K. A, PARL, F. F, WEITZMAN, S. A, GRAFF, J. G, HERMAN, J. G, DAVIDSON, N. E
Format: Article
Language:English
Subjects:
Biological and medical sciences
Gynecology. Andrology. Obstetrics
Mammary gland diseases
Medical sciences
Tumors
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Southern analysis has shown that DNA from 25% of primary estrogen receptor (ER) alpha -negative breast tumors displays aberrant methylation at one site within the ER gene CpG island. To examine more sites and increase sensitivity, we developed a methylation-specific PCR assay to map methylation of the entire ER CpG island. The island was unmethylated in normal breast tissue and ER-positive breast cancer cell lines, but extensively methylated in all ER-negative cell lines and breast tumors examined. In addition, some of the ER-positive/progesterone receptor-negative and ER-positive/progesterone receptor-positive tumors (about 70% and 35%, respectively) displayed methylation of the ER CpG island, suggesting that this heterogeneity within tumor cell populations could potentially shed light on the etiology of ER-negative recurrent tumors arising from ER-positive tumors.
ISSN:0008-5472
1538-7445