Ribosome-inactivating proteins and other lectins from Adenia (Passifloraceae)

The caudices of 10 Adenia species contain galactose-binding lectins that were purified by affinity chromatography. All lectins but three agglutinate human erythrocytes. Six lectins consist of two unequal chains, which can be separated by reduction, and inhibit protein synthesis both by a rabbit reti...

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Bibliographic Details
Published in:Toxicon (Oxford) 2005-11, Vol.46 (6), p.658-663
Main Authors: Pelosi, Emanuele, Lubelli, Chiara, Polito, Letizia, Barbieri, Luigi, Bolognesi, Andrea, Stirpe, Fiorenzo
Format: Article
Language:English
Subjects:
Adenine polynucleotide glycosylase
Animals
Biological and medical sciences
Cell Line
Chromatography, Affinity
Chromatography, High Pressure Liquid
Cytotoxicity Tests, Immunologic
Electrophoresis, Polyacrylamide Gel
Humans
Lectin
Medical sciences
Passifloraceae
Passifloraceae - chemistry
Plant Lectins - chemistry
Plant Lectins - isolation & purification
Plant poisons toxicology
Plants, Toxic - chemistry
Protein Synthesis Inhibitors - chemistry
Protein Synthesis Inhibitors - isolation & purification
Protein Synthesis Inhibitors - toxicity
Rabbits
Ribosome-inactivating protein
Ribosomes - drug effects
Species Specificity
Toxicology
Toxin
Toxins, Biological - chemistry
Toxins, Biological - isolation & purification
Toxins, Biological - toxicity
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Summary:The caudices of 10 Adenia species contain galactose-binding lectins that were purified by affinity chromatography. All lectins but three agglutinate human erythrocytes. Six lectins consist of two unequal chains, which can be separated by reduction, and inhibit protein synthesis both by a rabbit reticulocyte lysate and by HeLa and Raji cells. The lectins from A. goetzii, A. lanceolata and A. stenodactyla had the highest cytotoxicity, inhibiting cell protein synthesis with IC 50s (concentration inhibiting by 50%) below 0.1 ng/ml, and deadenylate DNA, thus being type 2 ribosome-inactivating proteins.
ISSN:0041-0101
1879-3150
DOI:10.1016/j.toxicon.2005.07.008