Genetic analyses of Asian seabass stocks using novel polymorphic microsatellites

To study genetic diversity of two cultured and one wild stock of Asian seabass ( Lates calcarifer) in South-East Asia and their relationships, thirty-six polymorphic microsatellites were isolated from partial genomic libraries. The average allele number of the 36 microsatellites present among 32 ind...

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Bibliographic Details
Published in:Aquaculture 2006-06, Vol.256 (1), p.167-173
Main Authors: Ze Yuan Zhu, Lin, Grace, Loong Chueng Lo, Yin Xue Xu, Feng, Felicia, Chou, Renee, Gen Hua Yue
Format: Article
Language:English
Subjects:
Agnatha. Pisces
alleles
Animal aquaculture
Animal productions
Aquaculture
Asian seabass
Biological and medical sciences
Dicentrarchus labrax
Fish
fish culture
Fundamental and applied biological sciences. Psychology
General aspects
Genetic diversity and broodstock
genetic polymorphism
genetic variation
Genetics
genome
Genomics
Lates calcarifer
mariculture
Marine
marine fish
microsatellite repeats
Microsatellites
Multiplex
Polymorphism
Vertebrates: general zoology, morphology, phylogeny, systematics, cytogenetics, geographical distribution
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Summary:To study genetic diversity of two cultured and one wild stock of Asian seabass ( Lates calcarifer) in South-East Asia and their relationships, thirty-six polymorphic microsatellites were isolated from partial genomic libraries. The average allele number of the 36 microsatellites present among 32 individuals, was 7.4/locus, ranging from 2 to 17. One multiplex PCR set of nine polymorphic microsatellites was used to genotype 294 individuals from three stocks. High levels of allelic and genetic diversity were observed in all three stocks (average allele number = 8.2/locus and H e = 0.75 in the wild stock; allele number = 9.6 and H e = 0.76 in Singapore stock I; and allele number = 8.0 and H e = 0.72 in Singapore stock II). F IS values within each stock were very low. Significant genetic differentiation ( F ST = 0.03, P < 0.05) and private alleles were detected among the three stocks. Thus, it may be possible to form a new superior broodstock with high genetic diversity by combining fish from different stocks. The developed polymorphic microsatellites, and especially the multiplex PCR set, are useful in monitoring genetic diversity of cultured stocks and for studying the genetic structure of wild stocks of Asian seabass.
ISSN:0044-8486
1873-5622
DOI:10.1016/j.aquaculture.2006.02.033