Stilbenoids from Rheum undulatum Protect Hepatocytes Against Oxidative Stress Through AMPK Activation

Oxidative stress promotes several diseases, including liver disease. We have isolated several stilbenoids from Rheum undulatum to investigate their hepatoprotective activities and mechanism. Stilbenoids from R. undulatum protects hepatocytes against arachidonic acid + iron (AA + Fe) induced oxidativ...

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Bibliographic Details
Published in:Phytotherapy research 2015-10, Vol.29 (10), p.1605-1609
Main Authors: Dong, Guang-zhi, Lee, Yu-ih, Jeong, Ji Hye, Zhao, Hui-Yuan, Jeon, Raok, Lee, Hwa Jin, Ryu, Jae-Ha
Format: Article
Language:English
Subjects:
AMP-Activated Protein Kinases - metabolism
AMPK
antioxidant
Arachidonic Acid - metabolism
Hep G2 Cells
hepatocyte protection
Hepatocytes - drug effects
Humans
Oxidative Stress - drug effects
Phosphorylation
Protective Agents - pharmacology
Rheum
Rheum undulatum
Stilbenes - pharmacology
stilbenoid
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Summary:Oxidative stress promotes several diseases, including liver disease. We have isolated several stilbenoids from Rheum undulatum to investigate their hepatoprotective activities and mechanism. Stilbenoids from R. undulatum protects hepatocytes against arachidonic acid + iron (AA + Fe) induced oxidative stress. Pterostilbene (compound 5) shows stronger activity than the others. Trimethoxystilbenoid (compound 6) shows best activity on protection of HepG2 cells from AA + Fe‐induced oxidative stress, and trans‐stilbenoid (compound 7) shows weak activity. These stilbenoids suppress ROS generation in AA + Fe‐treated HepG2 cells and also suppress AA + Fe‐induced MMP disruption. Their protective effects on AA + Fe‐induced MMP disruption were abrogated by treatment of AMP‐activated protein kinase (AMPK) inhibitor, compound C or transfection of dominant negative form of AMPK. Taken together, stilbenoids from R. undulatum protect hepatocytes against AA + Fe‐induced oxidative stress through AMPK activation. And the methoxy groups in the aryl groups are important for their cytoprotective activity. Copyright © 2015 John Wiley & Sons, Ltd.
ISSN:0951-418X
1099-1573
DOI:10.1002/ptr.5442