Fluorescence spectroscopy of high performance liquid chromatography fractionated marine and terrestrial organic materials

• Published in: Water research (Oxford) 1999-02, Vol.33 (2), p.512-520
• Main Authors: Lombardi, Ana T., Jardim, Wilson F.
• Format: Article
• Language: English
• Subjects: dissolved organic matter; Earth sciences; Earth, ocean, space; Exact sciences and technology; fluorescence; fulvic acid; Geochemistry; HPLC; humic substances; marine DOM; Soil and rock geochemistry
• ISSN: 0043-1354; 1879-2448
• DOI: 10.1016/S0043-1354(98)00233-4

Marine dissolved organic material (“marine DOM”, Mediterranean Sea) and a well characterized soil fulvic acid (“SFA”, Conway) were extracted using tC18 Sep-Pak cartridges. The extracts were characterized by fluorescence spectroscopy before and after reverse phase high performance liquid chromatographic fractionation (RP-HPLC). Based on soil fulvic acid, the tC18 Sep-Pak extraction procedure led to a loss of 65% of the fluorophores at λ ex 350 nm, and 34% of those at λ ex 450 nm, yet with no alteration in wavelength maxima; similar behavior was obtained for the marine DOM. Conventional fluorescence emission showed that the SFA fluorescence is 2.5 times higher than that of the marine DOM at λ ex/ λ em 350/452 nm and 11 times higher at λ ex/ λ em 450/522 nm. The higher fluorescence emission of the SFA in relation to the marine DOM can be attributed to compounds of high polarity. A blue shift was obtained when comparing the emission maximum of marine DOM ( λ em 437 nm) to that of SFA ( λ em 452 nm) at a fixed excitation of 350 nm, whereas no significant difference was obtained when excitation was fixed at 450 nm. Several distinct classes of fluorophores were detected in both marine DOM and SFA by the synchronous excitation technique: λ ex/ λ em 317/335 nm and 486/504 nm are present in the SFA, and λ ex/ λ em 352/370 nm and 566/584 nm in the marine DOM.