Production of poly-β-hydroxybutyrate on molasses by recombinant Escherichia coli

Beet molasses successfully replaced glucose as sole carbon source to produce poly- beta -hydroxybutyrate by a recombinant Escherichia coli strain (HMS174/pTZ18u-PHB). The fermentation with molasses was cheaper than with glucose. The final dry cell weight, PHB content and PHB productivity were 39.5 g...

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Bibliographic Details
Published in:Biotechnology letters 1998-04, Vol.20 (4), p.345-348
Main Authors: FANG LIU, WENQING LI, RIDGWAY, D, TINGYUE GU, ZHONGYAO SHEN
Format: Article
Language:English
Subjects:
Biological and medical sciences
Biotechnology
Escherichia coli
Fundamental and applied biological sciences. Psychology
Methods. Procedures. Technologies
Microbial engineering. Fermentation and microbial culture technology
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Summary:Beet molasses successfully replaced glucose as sole carbon source to produce poly- beta -hydroxybutyrate by a recombinant Escherichia coli strain (HMS174/pTZ18u-PHB). The fermentation with molasses was cheaper than with glucose. The final dry cell weight, PHB content and PHB productivity were 39.5 g/L, 80% (w/w) and 1 g/Lh, respectively, in a 5 L stirred tank fermenter after 31.5 h fed-batch fermentation with constant pH and dissolved O sub(2) content.
ISSN:0141-5492
1573-6776
DOI:10.1023/A:1005367011378