Developmental validation of the GlobalFiler® Express PCR Amplification Kit: A 6-dye multiplex assay for the direct amplification of reference samples

Highlights • Developmental validation following SWGDAM guidelines of the GlobalFiler® Express PCR Amplification Kit. • First 6-dye, 24-locus STR kit that contains all the “required” and “recommended” loci proposed by the CODIS Core Loci Working Group. • Direct amplification from reference samples wi...

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Bibliographic Details
Published in:Forensic science international : genetics 2015-11, Vol.19, p.148-155
Main Authors: Wang, Dennis Y, Gopinath, Siddhita, Lagacé, Robert E, Norona, Wilma, Hennessy, Lori K, Short, Marc L, Mulero, Julio J
Format: Article
Language:English
Subjects:
Animals
Coloring Agents - chemistry
Direct amplification
Forensic validation studies
GlobalFiler® Express Kit
Humans
Limit of Detection
Nucleotides - analysis
Pathology
Polymerase Chain Reaction - instrumentation
Polymerase Chain Reaction - methods
Rapid identification
Reproducibility of Results
Species Specificity
STR
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Summary:Highlights • Developmental validation following SWGDAM guidelines of the GlobalFiler® Express PCR Amplification Kit. • First 6-dye, 24-locus STR kit that contains all the “required” and “recommended” loci proposed by the CODIS Core Loci Working Group. • Direct amplification from reference samples with thermal cycling time under 45 min. • Concordance maintained between GlobalFiler® Express Kit and AmpFLSTR® Identifiler® Direct and NGM SElect™ Express Kits.
ISSN:1872-4973
1878-0326
DOI:10.1016/j.fsigen.2015.07.013