Population data of the GlobalFiler® Express loci in South Portuguese population

Abstract Allele frequencies and other relevant forensic parameters for 21 loci studied with GlobalFiler® Express amplification kit (Life Technologies) were calculated in a population of individuals residing in the south of Portugal. Blood stain samples were obtained from a total of 502 unrelated ind...

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Bibliographic Details
Published in:Forensic science international : genetics 2015-11, Vol.19, p.39-41
Main Authors: Almeida, Carina, Ribeiro, Teresa, Oliveira, Ana Rita, Porto, Maria João, Costa Santos, Jorge, Dias, Deodália, Dario, Paulo
Format: Article
Language:English
Subjects:
DNA - genetics
Gene Frequency
Genetics, Population
Globalfiler® express
Humans
Pathology
Polymerase Chain Reaction - instrumentation
Polymerase Chain Reaction - methods
Population genetics
Portugal
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Summary:Abstract Allele frequencies and other relevant forensic parameters for 21 loci studied with GlobalFiler® Express amplification kit (Life Technologies) were calculated in a population of individuals residing in the south of Portugal. Blood stain samples were obtained from a total of 502 unrelated individuals involved in paternity testing casework and directly PCR amplified with GlobalFiler® Express following manufacturer’s instructions. This kit comprises all the loci included in the extended European Standard Set (ESS) and in the Combined DNA Index System (CODIS), besides the very polymorphic D2S441, D19S433, and SE33. In our laboratory this is used as a screening tool to solve complex cases, as fatherless paternity tests or to help in paternity investigations where there is the need to study additional genetic markers. These studies are necessary to calculate statistical forensic parameters, such as power of discrimination or as power of exclusion. Statistical parameters including heterozigosity, homozigosity and combined power of exclusion were estimated.
ISSN:1872-4973
1878-0326
DOI:10.1016/j.fsigen.2015.06.001