Functional diversification of the dehydrin gene family in apple and its contribution to cold acclimation during dormancy

Dehydrins (DHN) are proteins involved in plant adaptive responses to abiotic stresses, mainly dehydration. Several studies in perennial crops have linked bud dormancy progression, a process characterized by the inability to initiate growth from meristems under favorable conditions, with DHN gene exp...

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Published in:Physiologia plantarum 2015-11, Vol.155 (3), p.315-329
Main Authors: Falavigna, Vítor da Silveira, Miotto, Yohanna Evelyn, Porto, Diogo Denardi, Anzanello, Rafael, Santos, Henrique Pessoa dos, Fialho, Flávio Bello, Margis-Pinheiro, Márcia, Pasquali, Giancarlo, Revers, Luís Fernando
Format: Article
Language:English
Subjects:
Acclimatization - genetics
Brazil
Cold Temperature
Evolution, Molecular
Gene expression
Gene Expression Regulation, Plant
Malus - genetics
Malus - physiology
Molecular Sequence Data
Multigene Family
Phylogeny
Plant Dormancy - genetics
Plant Proteins - genetics
Plant Proteins - metabolism
Real-Time Polymerase Chain Reaction
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Summary:Dehydrins (DHN) are proteins involved in plant adaptive responses to abiotic stresses, mainly dehydration. Several studies in perennial crops have linked bud dormancy progression, a process characterized by the inability to initiate growth from meristems under favorable conditions, with DHN gene expression. However, an in‐depth characterization of DHNs during bud dormancy progression is still missing. An extensive in silico characterization of the apple DHN gene family was performed. Additionally, we used five different experiments that generated samples with different dormancy status, including genotypes with contrasting dormancy traits, to analyze how DHN genes are being regulated during bud dormancy progression in apple by real‐time quantitative polymerase chain reaction (RT‐qPCR). Duplication events took place in the diversification of apple DHN family. Additionally, MdDHN genes presented tissue‐ and bud dormant‐specific expression patterns. Our results indicate that MdDHN genes are highly divergent in function, with overlapping levels, and that their expressions are fine‐tuned by the environment during the dormancy process in apple.
ISSN:0031-9317
1399-3054
DOI:10.1111/ppl.12338