Comparing different post-mortem human samples as DNA sources for downstream genotyping and identification

Highlights • DNA analysis of several samples from various post-mortem and environmental conditions. • Complete DNA profiles were generated from bone marrow, femur, metatarsal and patella. • Amplifiable DNA can be generated from bone marrow transferred on FTA® card. • Amplifiable target DNA maybe obt...

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Bibliographic Details
Published in:Forensic science international : genetics 2015-11, Vol.19, p.212-220
Main Authors: Calacal, Gayvelline C, Apaga, Dame Loveliness T, Salvador, Jazelyn M, Jimenez, Joseph Andrew D, Lagat, Ludivino J, Villacorta, Renato Pio F, Lim, Maria Cecilia F, Fortun, Raquel d.R, Datar, Francisco A, De Ungria, Maria Corazon A
Format: Article
Language:English
Subjects:
Alleles
Chromosomes, Human, Y
DNA
DNA - genetics
Forensic Anthropology
Genotype
Human identification
Humans
Mass disasters
Microsatellite Repeats - genetics
Pathology
Postmortem Changes
Putrefied remains
STR typing
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Summary:Highlights • DNA analysis of several samples from various post-mortem and environmental conditions. • Complete DNA profiles were generated from bone marrow, femur, metatarsal and patella. • Amplifiable DNA can be generated from bone marrow transferred on FTA® card. • Amplifiable target DNA maybe obtained using 0.1 ng DNA template. • 0.5 ng DNA template increased allele recovery and improved peak balance.
ISSN:1872-4973
1878-0326
DOI:10.1016/j.fsigen.2015.07.017