Construction of an integrated genetic map for Capsicum baccatum L

Capsicum baccatum L. is one of the five Capsicum domesticated species and has multiple uses in the food, pharmaceutical and cosmetic industries. This species is also a valuable source of genes for chili pepper breeding, especially genes for disease resistance and fruit quality. However, knowledge of...

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Bibliographic Details
Published in:Genetics and molecular research 2015-06, Vol.14 (2), p.6683-6694
Main Authors: Moulin, M M, Rodrigues, R, Ramos, H C C, Bento, C S, Sudré, C P, Gonçalves, L S A, Viana, A P
Format: Article
Language:English
Subjects:
Alleles
Capsicum - genetics
Capsicum annuum
Chromosome Mapping
Genetic Markers
Genome, Plant
Lod Score
Microsatellite Repeats
Polymorphism, Genetic
Polymorphism, Restriction Fragment Length
Quantitative Trait Loci
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Summary:Capsicum baccatum L. is one of the five Capsicum domesticated species and has multiple uses in the food, pharmaceutical and cosmetic industries. This species is also a valuable source of genes for chili pepper breeding, especially genes for disease resistance and fruit quality. However, knowledge of the genetic structure of C. baccatum is limited. A reference map for C. baccatum (2n = 2x = 24) based on 42 microsatellite, 85 inter-simple sequence repeat, and 56 random amplified polymorphic DNA markers was constructed using an F2 population consisting of 203 individuals. The map was generated using the JoinMap software (version 4.0) and the linkage groups were formed and ordered using a LOD score of 3.0 and maximum of 40% recombination. The genetic map consisted of 12 major and four minor linkage groups covering a total genome distance of 2547.5 cM with an average distance of 14.25 cM between markers. Of the 152 pairs of microsatellite markers available for Capsicum annuum, 62 were successfully transferred to C. baccatum, generating polymorphism. Forty-two of these markers were mapped, allowing the introduction of C. baccatum in synteny studies with other species of the genus Capsicum.
ISSN:1676-5680
1676-5680
DOI:10.4238/2015.June.18.12