MicroRNA-21 promotes proliferation of rat hepatocyte BRL-3A by targeting FASLG

• Published in: Genetics and molecular research 2015-04, Vol.14 (2), p.4150-4160
• Main Authors: Li, J J, Chan, W H, Leung, W Y, Wang, Y, Xu, C S
• Format: Article
• Language: English
• Subjects: 3' Untranslated Regions - genetics; Animals; Apoptosis - genetics; Base Sequence; Cell Line; Cell Proliferation - genetics; Fas Ligand Protein - genetics; Fas Ligand Protein - metabolism; Hepatectomy; Hepatocytes - metabolism; High-Throughput Nucleotide Sequencing; Liver Regeneration - physiology; MicroRNAs - genetics; Rats; Rats, Sprague-Dawley; Sequence Analysis, DNA
• ISSN: 1676-5680; 1676-5680
• DOI: 10.4238/2015.April.27.30

Rat liver regeneration (RLR) induced by partial hepatectomy involves cell proliferation regulated by numerous factors, including microRNAs (miRNAs). miRNA high-throughput sequencing has been established and used to analyze miRNA expression profiles. This study showed that 39 miRNAs were related to RLR through the analysis of miRNA high-throughput sequencing. Their role toward rat normal hepatocyte line BRL-3A was studied by gain- and loss-of-function analyses, and one of them, microRNA-21 (miR-21), obviously upregulated and promoted BRL-3A cell proliferation. Using bioinformatics to search for miR-21 targets revealed that Fas ligand (FASLG) is one of miR-21's target genes. A dual-luciferase report assay and Western blot assay showed that miR-21 directly targeted the 3'-untranslated region of FASLG and inhibited the expression of FASLG, which suggests that miR-21 promoted BRL-3A cell proliferation by reducing FASLG expression.