Nocardiopsis mangrovei sp. nov., isolated from mangrove sediment

• Published in: Antonie van Leeuwenhoek 2015-06, Vol.107 (6), p.1541-1546
• Main Authors: Huang, Hui-qin, Xing, Shan-shan, Yuan, Wei-dao, Wang, Ying, Liu, Min, Sun, Qian-guang, Lin, Xiang-zhi, Bao, Shi-xiang
• Format: Article
• Language: English
• Subjects: Actinobacteria - classification; Actinobacteria - genetics; Actinobacteria - isolation & purification; Actinobacteria - physiology; Bacteria; Bacterial Typing Techniques; Base Composition; Biomedical and Life Sciences; China; Cluster Analysis; Deoxyribonucleic acid; DNA; DNA, Bacterial - chemistry; DNA, Bacterial - genetics; DNA, Ribosomal - chemistry; DNA, Ribosomal - genetics; Geologic Sediments - microbiology; Hydrogen-Ion Concentration; Life Sciences; Medical Microbiology; Microbiology; Molecular Sequence Data; Nocardiopsis; Nucleic Acid Hybridization; Original Paper; Phylogenetics; Phylogeny; Plant Sciences; RNA, Ribosomal, 16S - genetics; Sequence Analysis, DNA; Sodium chloride; Sodium Chloride - metabolism; Soil microorganisms; Soil Science & Conservation; Taxonomy; Temperature
• ISSN: 0003-6072; 1572-9699
• DOI: 10.1007/s10482-015-0447-x

Two Gram-positive actinobacterial strains, designated HA11166 T and HA12420, were isolated from mangrove sediments in Hainan, China. The bacterial cells grew with 0–9 % (w/v) NaCl, at 15–40 °C and pH 5.0–10.0, with the optimum growth at 1 % NaCl, 30–37 °C and pH 7.0. The organisms had a range of chemical and morphological properties consistent with their classification in the genus Nocardiopsis . Phylogenetic analysis of the 16S rRNA gene sequences indicated that strains HA11166 T and HA12420 can be affiliated to the genus Nocardiopsis and most closely related to Nocardiopsis trehalosi VKM Ac-942 T (with the similarity of 97.2 and 97.5 %, respectively). The value of DNA–DNA relatedness between type strain HA11166 T , selected as the representative strain, and N. trehalosi VKM Ac-942 T was 38.8 %. The DNA G+C content of strain HA11166 T was 73.7 %. On the basis of these phenotypic and genotypic data, strains HA11166 T and HA12420 are proposed to represent a novel species of the genus Nocardiopsis , for which the name Nocardiopsis mangrovei sp. nov. is proposed. The type strain is HA11166 T (=CGMCC 4.7119 T =DSM 46665 T ).