Role of adiponectin in adipose tissue wound healing

The purpose of this study was to investigate the mechanism behind adipose tissue wound healing (ATWH). The preadipocyte cell line 3T3-L1 was cultured and expression of adiponectin receptors (AdipoR1/2) was detected by immunohistochemistry and reverse transcription polymerase chain reaction. The conc...

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Bibliographic Details
Published in:Genetics and molecular research 2015-08, Vol.14 (3), p.8883-8891
Main Authors: Jin, C E, Xiao, L, Ge, Z H, Zhan, X B, Zhou, H X
Format: Article
Language:English
Subjects:
3T3-L1 Cells
Adiponectin - biosynthesis
Adiponectin - metabolism
Adipose Tissue - injuries
Adipose Tissue - metabolism
Adipose Tissue - pathology
Animals
Cell Movement - physiology
Cell Proliferation - physiology
Enzyme-Linked Immunosorbent Assay
Mice
Receptors, Adiponectin - biosynthesis
Receptors, Adiponectin - metabolism
Wound Healing - physiology
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Summary:The purpose of this study was to investigate the mechanism behind adipose tissue wound healing (ATWH). The preadipocyte cell line 3T3-L1 was cultured and expression of adiponectin receptors (AdipoR1/2) was detected by immunohistochemistry and reverse transcription polymerase chain reaction. The concentration of adiponectin secreted at different cell densities was measured by enzyme-linked immunosorbent assay, while preadipocyte proliferation and migration were determined in vitro by MTT and wound closure assays. AdipoR1/2 were found to be expressed in 3T3-L1 preadipocytes. There were no statistically significant differences in the concentrations of adiponectin secreted by cell solutions of different densities (P > 0.05). In addition, adiponectin was seen to promote the growth and migration of preadipocytes. In conclusion, adiponectin may regulate ATWH by promoting preadipocyte proliferation and migration, and its systemic and/or local application is proposed as a promising therapeutic approach for the treatment of wounds incurred as a result of surgery.
ISSN:1676-5680
1676-5680
DOI:10.4238/2015.August.3.11