Analysis of ROP signaling in the leaf epidermis of mutant tomato with low-energy ion beam

The importance of the ROP small GTPase signaling pathway in the regulation of cellular polarity growth in eukaryotes has been thoroughly studied. In this study, we examined the LeROP small GTPase (related to Arabidopsis thaliana genome LeROP GTPase in tomato) signaling of cell polarity growth in the...

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Bibliographic Details
Published in:Genetics and molecular research 2015-04, Vol.14 (2), p.3807-3816
Main Authors: Liang, Q X, Cao, G Q, Zhao, S P, Huang, Q C, Ying, F Q, Chen, W
Format: Article
Language:English
Subjects:
Arabidopsis
Arabidopsis thaliana
Base Sequence
Gene Expression
GTP-Binding Proteins - metabolism
Lycopersicon esculentum
Lycopersicon esculentum - cytology
Lycopersicon esculentum - metabolism
Lycopersicon esculentum - radiation effects
Phylogeny
Plant Epidermis - cytology
Plant Epidermis - metabolism
Plant Epidermis - radiation effects
Plant Proteins - metabolism
Sequence Analysis, DNA
Signal Transduction
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Summary:The importance of the ROP small GTPase signaling pathway in the regulation of cellular polarity growth in eukaryotes has been thoroughly studied. In this study, we examined the LeROP small GTPase (related to Arabidopsis thaliana genome LeROP GTPase in tomato) signaling of cell polarity growth in the mutant (M-1) tomato. Interestingly, we detected expansive growth of epidermis cells in M-1, in which the leaves appeared slightly lobed shaped. However, we observed jigsaw puzzle shaped and deeply lobed shaped leaves in wild-type leaf epidermis cells. The t-test showed significant difference (P < 0.05). Based on previous studies of the AtROP gene in Arabidopsis leaf epidermis cells, we hypothesized that the growth of mutant M-1 tomato leaf epidermis cell is related to AtROP gene signal transmission. The results of reverse transcription-polymerase chain reaction showed the expression of LeROP2, LeROP4, and LeROP7 in M-1 mutants were stronger than in wild-type cells. At the flowering stage, LeROP2 GTPase showed no expression in wild-type cells, but was expressed in mutant cells. This study revealed a link between the low-energy ion beam and the ROP GTPase signaling pathway in tomato. In addition, the ROP gene changes analyzed suggest a new mechanism for mutations following low-energy ion beam implantation.
ISSN:1676-5680
1676-5680
DOI:10.4238/2015.April.22.10