Orientation of a Dip-Coated Bacteriorhodopsin Thin Film Studied by Second Harmonic Generation Interferometry

We investigated the absolute orientation of bacteriorhodopsin (bR) thin film prepared by the dip-coating technique by means of second harmonic generation (SHG) interferometry. bRs in the bR thin films prepared in this technique tend to have a preference to orient with the cytoplasmic side away from...

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Bibliographic Details
Published in:Japanese Journal of Applied Physics 2013-05, Vol.52 (5), p.05DB03-05DB03-5
Main Authors: Yamada, Toshiki, Haruyama, Yoshihiro, Kasai, Katsuyuki, Terui, Toshifumi, Tanaka, Shukichi, Kaji, Takahiro, Kikuchi, Hiroshi, Otomo, Akira
Format: Article
Language:English
Subjects:
Bacteria
Bacteriorhodopsin
Covalence
Glass
Interferometry
Mathematical analysis
Orientation
Second harmonic generation
Thin films
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Summary:We investigated the absolute orientation of bacteriorhodopsin (bR) thin film prepared by the dip-coating technique by means of second harmonic generation (SHG) interferometry. bRs in the bR thin films prepared in this technique tend to have a preference to orient with the cytoplasmic side away from the glass substrate and extracellular side toward the glass substrate. To assist in the analysis, the hyperpolarizability of covalently bound retinal chromophore was also evaluated by a computational calculation.
ISSN:0021-4922
1347-4065
DOI:10.7567/JJAP.52.05DB03