Experimental application of Lactobacillus fermentum CCM 7421 in combination with chlorophyllin in dogs

Chlorophyll belongs in a larger class of phytochemical plant pigments currently receiving more attention as a physiologically active dietary component. Although most research has focused on its biological activities such as its antioxidant, antimutagenic, anti-inflammatory or apoptotic effects in hu...

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Bibliographic Details
Published in:Applied microbiology and biotechnology 2015-10, Vol.99 (20), p.8681-8690
Main Authors: Strompfova, Viola, Kubasova, Ivana, Farbakova, Jana, GancarAeikova, Soa, Mudroova, Dagmar, MaAeari, Aladar, Laukova, Andrea
Format: Article
Language:English
Subjects:
Acids
Additives
Analysis
Animals
Anti-Bacterial Agents - administration & dosage
Anti-Bacterial Agents - pharmacology
anti-infective properties
Antimicrobial agents
antioxidant activity
apoptosis
Applied Microbial and Cell Physiology
Bacteria
Biomarkers - analysis
Biomedical and Life Sciences
Biota - drug effects
Biotechnology
Blood Chemical Analysis
Chlorophyll
Chlorophyllides - administration & dosage
Chlorophyllides - pharmacology
Citrobacter freundii
coliform bacteria
Copper
Diet
Dietary Supplements
Dogs
Experiments
feces
Feces - microbiology
Fermentation
growth retardation
Hematology
humans
in vivo studies
indicator species
Lactobacillus fermentum
Lactobacillus fermentum - drug effects
Lactobacillus fermentum - growth & development
leukocytes
Life Sciences
Listeria monocytogenes
Medical research
Microbial Genetics and Genomics
Microbiology
Microbiota
Organic acids
Pet food
phagocytosis
Pharmacy
Physiology
plant pigments
Probiotics
Probiotics - administration & dosage
propionic acid
rodents
Staphylococcus
Studies
Treatment Outcome
Veterinary medicine
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Summary:Chlorophyll belongs in a larger class of phytochemical plant pigments currently receiving more attention as a physiologically active dietary component. Although most research has focused on its biological activities such as its antioxidant, antimutagenic, anti-inflammatory or apoptotic effects in humans or rodents, there is limited knowledge at this time about the combinative possibilities of chlorophyll with probiotic bacteria. Our aim was to test the growth characteristics of canine-derived probiotic strain Lactobacillus fermentum CCM 7421 in the presence of different concentrations of chlorophyllin in vitro. Antimicrobial activity of chlorophyllin against canine indicator bacteria was also detected. In the in vivo study, chlorophyllin, L. fermentum CCM 7421 and the combination of both additives on faecal microbiota, faecal organic acid concentrations, haematological and immunological parameters in dogs were tested. Forty dogs were divided into 4 treatment groups; control (C); receiving chlorophyllin (60 mg/day/dog, CH group); L. fermentum CCM 7421 (10⁸ CFU/day/dog, LF group); and both additives (CH + LF group), 10 dogs in each group. The experiment lasted for 28 days with a 14-day treatment period (sample collection at days 0, 7, 14 and 28). Results showed no growth inhibition of strain CCM 7421 by 0.05–0.25 % of chlorophyllin in broth after 24 h. Reduced growth of staphylococci, Listeria monocytogenes and Citrobacter freundii was observed at 1 % chlorophyllin (P 
ISSN:0175-7598
1432-0614
DOI:10.1007/s00253-015-6724-9