Comparison of selected methods for recovery of Giardia spp. cysts and Cryptosporidium spp. oocysts in wastewater

More precise methods are needed to recover Giardia and Cryptosporidium (oo)cysts from wastewater in order to advance research related to their inactivation, removal, quantification, and species differentiation. This study applied different methods to recover the maximum number of (oo)cysts from wast...

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Bibliographic Details
Published in:Journal of water and health 2015-09, Vol.13 (3), p.811-818
Main Authors: Medeiros, Raphael Corrêa, Daniel, Luiz Antonio
Format: Article
Language:English
Subjects:
Centrifugation
Centrifuging
Cryptosporidium
Cryptosporidium - isolation & purification
Cryptosporidium parvum
Cysts
Deactivation
Developing countries
Differentiation
Effluent treatment
Effluents
Environmental protection
Epidemics
Filtration
Giardia
Giardia - isolation & purification
Handling
Homogenization
Immunomagnetic Separation
Inactivation
LDCs
Membrane filtration
Methods
Oocysts
Oocysts - cytology
Parasites
Protozoa
Raw wastewater
Recovering
Recovery
Removal
Risk factors
Sludge
Turbidity
Waste Water - parasitology
Wastewater
Wastewater treatment
Water Purification - methods
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Summary:More precise methods are needed to recover Giardia and Cryptosporidium (oo)cysts from wastewater in order to advance research related to their inactivation, removal, quantification, and species differentiation. This study applied different methods to recover the maximum number of (oo)cysts from wastewater samples using ColorSeed®. Immunomagnetic separation assisted in capturing oocysts mainly in samples with medium and low turbidity. A triple centrifugation method reached recovery rates of 85% and 20%, for Giardia cysts and Cryptosporidium oocysts, respectively, in raw wastewater, and 62.5 and 17.5% in secondary-treated effluent. For low turbidity-treated effluent, membrane filtration reached 67.5% recovery for Giardia cysts and 22.5% for Cryptosporidium oocysts. Simple, quick and low-cost methods do not involve much handling of the samples and could be useful, particularly in developing countries.
ISSN:1477-8920
1996-7829
DOI:10.2166/wh.2015.228