A Method for the Simultaneous Detection of Mast Cells and Nerve Terminals in the Thymus in Laboratory Mammals

The aim of the present work was to develop a method for the simultaneous detection of mast cells (MC) and nerve fiber terminals based on methods developed for the histochemical identification of MC with Alcian blue and an immunochemical reaction for synaptophysin. The protocol developed here provide...

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Bibliographic Details
Published in:Neuroscience and behavioral physiology 2015-05, Vol.45 (4), p.371-374
Main Authors: Gusel’nikova, V. V., Sukhorukova, E. G., Fedorova, E. A., Polevshchikov, A. V., Korzhevskii, D. E.
Format: Article
Language:English
Subjects:
Antibodies
Behavioral Sciences
Biomedical and Life Sciences
Biomedicine
Ethanol
Laboratory animals
Methods
Morphology
Nervous system
Neurobiology
Neurosciences
Reagents
Thymus gland
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Summary:The aim of the present work was to develop a method for the simultaneous detection of mast cells (MC) and nerve fiber terminals based on methods developed for the histochemical identification of MC with Alcian blue and an immunochemical reaction for synaptophysin. The protocol developed here provides highly selective and reproducible simultaneous detection of MC and nerve endings on paraffin sections of thymus from laboratory mammals. The method can be used both for detecting the spatial interactions of MC and nerve terminals and for independent studies of the innervation of the internal organs of mammals. The optimum fixative was zinc-ethanol-formaldehyde.
ISSN:0097-0549
1573-899X
DOI:10.1007/s11055-015-0084-x