Characteristics of Postsynaptic Currents in Primary Cultures of Rat Cerebral Cortical Neurons

The features of the generation of postsynaptic currents in primary cultures of cortical neurons from rat brains were studied at 7–20 days in vitro (DIV). Use of specific blockers of postsynaptic ion channels showed that from 10 DIV, neurons generated all types of electrical activity typical of the a...

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Bibliographic Details
Published in:Neuroscience and behavioral physiology 2015-05, Vol.45 (4), p.431-439
Main Authors: Sibarov, D. A., Antonov, S. M.
Format: Article
Language:English
Subjects:
Behavioral Sciences
Biomedical and Life Sciences
Biomedicine
Brain
Embryos
Neural networks
Neurobiology
Neurons
Neurosciences
Physiology
Prenatal development
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Summary:The features of the generation of postsynaptic currents in primary cultures of cortical neurons from rat brains were studied at 7–20 days in vitro (DIV). Use of specific blockers of postsynaptic ion channels showed that from 10 DIV, neurons generated all types of electrical activity typical of the adult cortex: spontaneous miniature inhibitory (mIPSP) and excitatory (mEPSP) currents, as well as giant excitatory currents and action potentials (AP). The frequency of mEPSP increased exponentially from DIV 7 to 20 in parallel with a change in the nature of AP generation. mEPSP generated by activation of NMDA and AMPA or only AMPA receptors were identified. The role of inhibition of presynaptic NMDA receptors by magnesium ions or AP5 in modulating the frequency and amplitude of mEPSP was demonstrated; this was a characteristic of neurons cultured from brain slices and appeared to linked with the lack of glial control of synaptic transmission.
ISSN:0097-0549
1573-899X
DOI:10.1007/s11055-015-0093-9