Analysis of preference for carbon source utilization among three strains of aromatic compounds degrading Pseudomonas

• Published in: FEMS microbiology letters 2015-10, Vol.362 (20), p.fnv139
• Main Authors: Karishma, M., Trivedi, Vikas D., Choudhary, Alpa, Mhatre, Akanksha, Kambli, Pranita, Desai, Jinal, Phale, Prashant S.
• Format: Article
• Language: English
• Subjects: Aromatic compounds; Benzoates; Benzoates - metabolism; Biodegradation, Environmental; Bioremediation; Carbaryl; Carbon sources; Catechol; Depletion; Ferulic acid; Glucose; Glucose - metabolism; Growth patterns; Isomers; Microbiology; Naphthalene; Organic acids; Phthalates; Phthalic Acids - metabolism; Protocatechuate 3,4-dioxygenase; Pseudomonas; Pseudomonas - growth & development; Pseudomonas - metabolism; Pseudomonas aeruginosa; Pseudomonas putida; Pseudomonas putida - growth & development; Pseudomonas putida - metabolism; Soil isolates; Succinates - metabolism; Utilization; Vanillic acid; Vanillin; Veratraldehyde
• ISSN: 1574-6968; 0378-1097; 1574-6968
• DOI: 10.1093/femsle/fnv139

Soil isolates Pseudomonas putida CSV86, Pseudomonas aeruginosa PP4 and Pseudomonas sp. C5pp degrade naphthalene, phthalate isomers and carbaryl, respectively. Strain CSV86 displayed a diauxic growth pattern on phenylpropanoid compounds (veratraldehyde, ferulic acid, vanillin or vanillic acid) plus glucose with a distinct second lag-phase. The glucose concentration in the medium remained constant with higher cell respiration rates on aromatics and maximum protocatechuate 3,4-dioxygenase activity in the first log-phase, which gradually decreased in the second log-phase with concomitant depletion of the glucose. In strains PP4 and C5pp, growth profile and metabolic studies suggest that glucose is utilized in the first log-phase with the repression of utilization of aromatics (phthalate or carbaryl). All three strains utilize benzoate via the catechol ‘ortho’ ring-cleavage pathway. On benzoate plus glucose, strain CSV86 showed preference for benzoate over glucose in contrast to strains PP4 and C5pp. Additionally, organic acids like succinate were preferred over aromatics in strains PP4 and C5pp, whereas strain CSV86 co-metabolizes them. Preferential utilization of aromatics over glucose and co-metabolism of organic acids and aromatics are found to be unique properties of P. putida CSV86 as compared with strains PP4 and C5pp and this property of strain CSV86 can be exploited for effective bioremediation. The current work investigates the uniqueness of Pseudomonas putida CSV86 for its preference for aromatic compounds over simple carbon source in comparison with other Pseudomonas.