Erratum to “Metal donation and apo-metalloenzyme activation by stable isotopically labeled metallothionein” [Marine Environmental Research 58 (2004) 371–375]

• Published in: Marine environmental research 2005-07, Vol.60 (1), p.131-132
• Main Authors: Mason, Andrew Z., Perico, Natalie, Moeller, Rhonda, Thrippleton, Kelly, Potter, Tiffany, Lloyd, Douglas
• Format: Article
• Language: English
• Subjects: Marine
• ISSN: 0141-1136; 1879-0291
• DOI: 10.1016/j.marenvres.2004.06.002

The publisher regrets that errors appeared in the last line of the abstract and the caption to Fig. 1. The text is now reproduced correctly below. Abstract Coupled HPLC-ICP-MS has been used to quantitatively study the effects of GSSG and GSH on the ability of metallothionein (MTII) to donate essential and non-essential metals to apo-carbonic anhydrase. Stable isotopically labeled super(67)Zn sub(3)Cd sub(4) MTII was used to enable Zn donated from MTII to be differentiated from extraneous sources of Zn. Transfer of both super(67)Zn and Cd from MTII to apo-carbonic anhydrase was noted in the absence of either GSSG or GSH. GSSG increased the initial transfer of both Zn and Cd. Thereafter, a gradual increase in the super(67)Zn content at the expense of Cd was noted over 24-h indicating continued interaction and exchange between MTII and the enzyme commensurate with the relative preferences shown by the proteins for these two metals. Although GSH also increased transfer of super(67)Zn from MT it reduced the simultaneous transfer of Cd to the enzyme thereby conferring protection against Cd induced inactivation. Fig. 1. Representative ion exchange HPLC-ICP-MS elemental profiles showing the transfer of super(67)Zn and super(114)Cd to apo-CA (4.09 min) from super(67)Zn sub(3)Cd sub(4)MTII (11.05 min) in the absence (a) and presence of GSH (b) and GSSG (c). Incubations were for 1 h at 25 degree C. The mixtures applied to the column contained a total of 25 pmol of super(67)Zn sub(3)Cd sub(4)MTII, 175 pmol of apo-CA in the presence or absence of 25 nmol of GSH or GSSG in a total volume of 50 mu l. The peak at 2.6 min eluted with the void and was tentatively identified as an oxidation product of MTII since the addition of GSSG increased (b), while GSH abolished (c), this peak.