Phylogeny of Vicia subgenus Vicia (Fabaceae) based on analysis of RAPDs and RFLP of PCR-amplified chloroplast genes

We report the results of two methods of DNA analysis to elucidate phylogenetic relationships among 29 Vicia subgen. Vicia species in comparison with two species of subgenus Vicilla sect. Vicilla. The methods employed were RAPD analysis of total genomic DNA and PCR-RFLP analysis of five chloroplast g...

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Bibliographic Details
Published in:Genetic resources and crop evolution 1999-04, Vol.46 (2), p.149-161
Main Authors: Potokina, E. (Vavilov Inst. of Plant Industry, St. Petersburg (Russian Federation). Dept. of Plant Introduction and Taxonomy), Tomooka, N, Vaughan, D.A, Alexandrova, T, Xu, R.Q
Format: Article
Language:English
Subjects:
Chloroplasts
Deoxyribonucleic acid
DNA
EVOLUCION
EVOLUTION
Ferns
GENE
GENES
GENETIC RESOURCES
PCR
Phylogeny
Polymerase chain reaction
Random amplified polymorphic DNA
RAPD
RECURSOS GENETICOS
RESSOURCE GENETIQUE
Restriction fragment length polymorphism
RFLP
RpoB protein
Species
TAXONOMIA
TAXONOMIE
TAXONOMY
VICIA
Vicia anatolica
Vicia bithynica
Vicia faba
Vicia hyrcanica
Vicia lutea
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Summary:We report the results of two methods of DNA analysis to elucidate phylogenetic relationships among 29 Vicia subgen. Vicia species in comparison with two species of subgenus Vicilla sect. Vicilla. The methods employed were RAPD analysis of total genomic DNA and PCR-RFLP analysis of five chloroplast genes, rbcL, rpoB, 16S, psaA and trnK. The results of each method were similar and complementary, and support the current taxonomic systems of subsp. Vicia. According to RAPD and PCR-RFLP analysis the Narbonensis complex can be considered a well separated section, which may be related to section Vicia. Sections Vicia, Atossa and Wiggersia are separate, but closely related sections. Species of the section Hypechusa form a single monophyletic section, where V. lutea, V. anatolica and V. hyrcanica are quite remote from other species. Our results suggest that within the subgenus Vicia, V. faba is more closely related to V. bithynica and that these two species are most closely related to section Peregrinae. We found that PCR-RFLP of cp DNA provided more precise information concerning relationships between Vicia sections than RAPD analysis. However, RAPD analysis was more informative concerning diversity of closely related Vicia taxa, such as the variable groups, section Narbonensis and V. sativa aggregate.
ISSN:0925-9864
1573-5109
DOI:10.1023/A:1008640322198