Troxerutin, a plant flavonoid, protects cells against oxidative stress-induced cell death through radical scavenging mechanism

•TRX scavenged biologically relevant and model radicals.•TRX exhibited high rate constant with hydroxyl radicals.•It inhibited peroxyl radical-induced damage to DNA, lipids and proteins.•It also protected cells against oxidative stress induced cell death. Troxerutin (TRX) is a flavonoid present in t...

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Bibliographic Details
Published in:Food chemistry 2016-03, Vol.194, p.32-45
Main Authors: Panat, Niranjan A., Maurya, Dharmendra K., Ghaskadbi, Saroj S., Sandur, Santosh K.
Format: Article
Language:English
Subjects:
Antioxidant
Antioxidants - chemistry
Cell Death
Flavonoids
Free radicals
Free Radicals - chemistry
Hydroxyethylrutoside - analogs & derivatives
Hydroxyethylrutoside - chemistry
Oxidative Stress
Plant Extracts - chemistry
Pulse radiolysis
Reactive Oxygen Species - chemistry
ROS
Troxerutin
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Summary:•TRX scavenged biologically relevant and model radicals.•TRX exhibited high rate constant with hydroxyl radicals.•It inhibited peroxyl radical-induced damage to DNA, lipids and proteins.•It also protected cells against oxidative stress induced cell death. Troxerutin (TRX) is a flavonoid present in tea, coffee, cereal grains, various fruits and vegetables have been reported to exhibit radioprotective, antithrombotic, nephro and hepato-protective effects. A systematic study was undertaken to evaluate its free radical scavenging ability and anti-apoptotic activity in cell-free and cellular systems. TRX scavenged superoxide, nitric oxide and also other model stable radicals like 1,1-diphenyl-2-picryl-hydazyl, and 2,2′-azinobis3-ethylbenzothiazoline-6-sulfonic acid. It reacted with hydroxyl radicals, carbonate and thiocyanate anions, as evaluated by pulse radiolysis and stopped flow techniques. TRX protected different cell types (epithelial cells, fibroblasts and lymphocytes) against peroxyl radical-induced apoptosis, necrosis and mitotic death. It scavenged intracellular basal and inducible ROS levels and also restored depletion of intracellular GSH levels, suggesting that free radical scavenging ability may be responsible for the observed cytoprotection of different cell types. TRX may find application as an adjuvant in treating various diseases attributed to oxidative stress.
ISSN:0308-8146
1873-7072
DOI:10.1016/j.foodchem.2015.07.078