MicroRNA-142-3p inhibits cell proliferation and invasion of cervical cancer cells by targeting FZD7

MicroRNAs (miRNAs) are a class of small noncoding RNAs that play important roles in tumorigenesis and tumor progression through regulation of gene expression. Earlier, miR-142-3p was shown to decreased in cervical cancer cells; here; we explore the biological functional role of miR-142-3p and underl...

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Bibliographic Details
Published in:Tumor biology 2015-09, Vol.36 (10), p.8065-8073
Main Authors: Deng, Boya, Zhang, Yi, Zhang, Siyang, Wen, Fang, Miao, Yuan, Guo, Kejun
Format: Article
Language:English
Subjects:
Apoptosis
Biomarkers, Tumor - genetics
Biomarkers, Tumor - metabolism
Biomedical and Life Sciences
Biomedicine
Blotting, Western
Cancer Research
Cell Movement
Cell Proliferation
Cervical cancer
Female
Frizzled Receptors - genetics
Frizzled Receptors - metabolism
Gene expression
Humans
MicroRNAs
MicroRNAs - genetics
Neoplasm Invasiveness
Real-Time Polymerase Chain Reaction
Research Article
Reverse Transcriptase Polymerase Chain Reaction
RNA, Messenger - genetics
Tumor Cells, Cultured
Tumors
Uterine Cervical Neoplasms - genetics
Uterine Cervical Neoplasms - metabolism
Uterine Cervical Neoplasms - pathology
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Summary:MicroRNAs (miRNAs) are a class of small noncoding RNAs that play important roles in tumorigenesis and tumor progression through regulation of gene expression. Earlier, miR-142-3p was shown to decreased in cervical cancer cells; here; we explore the biological functional role of miR-142-3p and underlying mechanism in cervical cancer cells. We first detected the expression of miR-142-3p in six human cervical cancer cell lines and chose HeLa and SiHa cells for functional studies. By gain and loss of function experiments, we showed that overexpression of miR142-3p resulted in downregulation of Frizzled7 receptor (FZD7) and inhibited proliferation and invasion in HeLa and SiHa cells, whereas miR142-3p inhibitor-transfected cells showed reduced FZD7 expression and increased invasion capacity. In addition, we demonstrated that FZD7 was a direct target of miR-142-3p by dual luciferase assay and Western blot analysis. Overexpression of FZD7 expression was able to reverse the inhibitory effects induced by miR-142-3p. Taken together, miR-142-3p functions tumor suppressive effects in cell proliferation and invasion in cervical cancer cells, suggesting a potential therapeutic approach for cervical cancer.
ISSN:1010-4283
1423-0380
DOI:10.1007/s13277-015-3483-2