Arabidopsis thaliana dhdps gene encoding dihydrodipicolinate synthase, key enzyme of lysine biosynthesis, is expressed in a cell-specific manner

• Published in: Plant molecular biology 1999-03, Vol.39 (4), p.695-708
• Main Authors: Vauterin, M, Frankard, V, Jacobs, M
• Format: Article
• Language: English
• Subjects: Amino Acid Sequence; Amino acids; Arabidopsis - cytology; Arabidopsis - enzymology; Arabidopsis - genetics; Arabidopsis thaliana; Base Sequence; beta-glucuronidase; Biosynthesis; Cells, Cultured; Cloning, Molecular; Cotyledon - enzymology; enzymes; flowers; genbank/x98080; gene expression; Gene Expression Regulation, Enzymologic; Gene Expression Regulation, Plant; Genes; Genes, Plant; Glucuronidase - biosynthesis; histochemistry; Hydro-Lyases - chemistry; Hydro-Lyases - genetics; Hydro-Lyases - metabolism; leaves; Lysine - biosynthesis; meristems; messenger RNA; Molecular Sequence Data; Nicotiana; Nicotiana tabacum; nucleotide sequences; Plant Roots - enzymology; plant vascular system; Plants, Toxic; Pollen; promoter regions; Promoter Regions, Genetic; Protoplasts - enzymology; recombinant DNA; Recombinant Fusion Proteins - biosynthesis; Recombinant Fusion Proteins - chemistry; Recombinant Fusion Proteins - metabolism; reporter genes; Restriction Mapping; root meristems; shoot meristems; stems; structural genes; Transcription, Genetic; transgenic plants
• ISSN: 0167-4412; 1573-5028
• DOI: 10.1023/A:1006132428623

Lysine synthesis in prokaryotes, some phycomycetes and higher plants starts with the condensation of L-aspartate-beta-semialdehyde (L-ASA) and pyruvate into dihydrodipicolinic acid. The enzyme that catalyses this step, dihydrodipicolinate synthase (DHDPS), is inhibited by the end-product lysine and is therefore thought to have a regulatory control on lysine synthesis. We have cloned and sequenced an Arabidopsis thaliana DNA fragment containing 900 bases upstream of the dhdps coding sequence. A transcriptional fusion of this fragment with the beta-glucuronidase reporter gene (uidA, Gus) was used to study the transcription properties of this promoter fragment (DS). No lysine-induced repression on transcription could be detected. Expression of DS-Gus activity in transformed Arabidopsis thaliana and Nicotiana tabacum was found to be cell type-specific. In the vegetative parts of the plant, GUS activity was located in meristems and young vasculature of roots, in vasculature of stem and leaves and in the meristems of young shoots. In flowers, high expression was found in the carpels, style, stigma, developing embryos, tapetum of young anthers and pollen. We demonstrated that the Arabidopsis DS promoter can direct its cell type-specific expression in a heterologous plant, Nicotiana tabacum. The importance of transcriptional regulation of the dhdps gene, and in more general genes involved in amino acid biosynthesis, is discussed.