Characterization of temperature-sensitive strains of Neospora caninum in mice

Temperature-sensitive (ts) strains of the Neospora caninum tachyzoites were selected by chemical mutagenesis and selection for growth at 32 C. Three ts strains and the parental, N. caninum wild-type strain, NC-1, were examined in the present study for their ability to cause disease in inbred BALB/c...

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Bibliographic Details
Published in:The Journal of parasitology 1999-02, Vol.85 (1), p.64-67
Main Authors: Lindsay, D.S, Lenz, S.D, Blagburn, B.L, Brake, D.A
Format: Article
Language:English
Subjects:
ambient temperature
Animals
Antibodies
Antibodies, Protozoan - blood
Biological and medical sciences
Coccidiosis - immunology
Coccidiosis - parasitology
Coccidiosis - prevention & control
disease models
Disease Models, Animal
Encephalitis - immunology
Encephalitis - parasitology
Encephalitis - prevention & control
Female
Fundamental and applied biological sciences. Psychology
immunity
Immunosuppression
Lesions
Life cycle. Host-agent relationship. Pathogenesis
Life Cycles-Survey
live vaccines
Memory interference
Mice
Mice, Inbred BALB C
Mortality
mutants
Neospora - immunology
Neospora - pathogenicity
Neospora caninum
neosporosis
Parasites
Parasitology
pathogenicity
Protozoa
protozoal infections
Protozoan Vaccines - standards
strains
Tachyzoites
Temperature
Vaccination
vaccine development
Veterinary medicine
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Summary:Temperature-sensitive (ts) strains of the Neospora caninum tachyzoites were selected by chemical mutagenesis and selection for growth at 32 C. Three ts strains and the parental, N. caninum wild-type strain, NC-1, were examined in the present study for their ability to cause disease in inbred BALB/c mice, outbred ICR mice, and chemically immunosuppressed ICR mice. In BALB/c mice, all 3 strains failed to induce clinical disease, whereas infection with the NC-1 strain caused central nervous system disease and death in some mice. No disease was observed in ICR mice inoculated with the 3 ts strains or the NC-1 strain. All immunosuppressed ICR mice inoculated with the NC-1 strain died, whereas no immunosuppressed mice inoculated with the NCts-4 strain and only 1 of 5 mice inoculated with the NCts-8 and NCts-12 strains died. The NCts-4 and NCts-12 strains reverted to a wild-type phenotype when grown at 37 C. Vaccination of BALB/c mice with live, but not frozen NCts-8 strain tachyzoites induced significant (P < 0.05) protection following NC-1 strain challenge.
ISSN:0022-3395
1937-2345
DOI:10.2307/3285701