Sporozoite and Liver Stage Antigen Plasmodium falciparum peptides bind specifically to human hepatocytes

Sporozoite and Liver Stage Antigen (SALSA) sequence synthetic peptides were used in HepG2 cell binding assays to identify regions involved in parasite invasion. SALSA 20608 ( 21 IWASEKKDEKEASEQGEESHY 40 ) and 20611 ( 64 KKDDGTDKVQEKVLEKSPKY 83 ) peptides were determined as having high binding activi...

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Bibliographic Details
Published in:Vaccine 2004-03, Vol.22 (9), p.1150-1156
Main Authors: Puentes, Alvaro, Garcı́a, Javier, Vera, Ricardo, López, Ramsés, Suarez, Jorge, Rodrı́guez, Luis, Curtidor, Hernando, Ocampo, Marisol, Tovar, Diana, Forero, Martha, Bermudez, Adriana, Cortes, Jimena, Urquiza, Mauricio, Patarroyo, Manuel E.
Format: Article
Language:English
Subjects:
Amino Acid Sequence
Amino Acid Substitution
Animals
Antigens, Protozoan - immunology
Antigens, Protozoan - metabolism
Aotus trivirgatus
Applied microbiology
Binding peptides
Binding, Competitive
Biological and medical sciences
Cell Line
Critical residues
Electrophoresis, Polyacrylamide Gel
Erythrocytes - parasitology
Fluorescent Antibody Technique, Indirect
Fundamental and applied biological sciences. Psychology
Hepatocyte binding peptides
Hepatocytes - metabolism
Hepatocytes - parasitology
Humans
Isotope Labeling
Liver - parasitology
Microbiology
Molecular Sequence Data
Peptides - chemical synthesis
Peptides - metabolism
Plasmodium falciparum
Plasmodium falciparum - immunology
Plasmodium falciparum - metabolism
Protein Binding
SALSA protein
Sporozoites - immunology
Vaccines, antisera, therapeutical immunoglobulins and monoclonal antibodies (general aspects)
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Description
Summary:Sporozoite and Liver Stage Antigen (SALSA) sequence synthetic peptides were used in HepG2 cell binding assays to identify regions involved in parasite invasion. SALSA 20608 ( 21 IWASEKKDEKEASEQGEESHY 40 ) and 20611 ( 64 KKDDGTDKVQEKVLEKSPKY 83 ) peptides were determined as having high binding activity in HepG2 cell assays, some of them were located in immunogenic regions. Immune-fluorescence antibody test with 24276 (20608 peptide analogue, CGIWS SM K M DEK M A AM QGEESHCG) showed sporozoite and merozoite reactivity. This data suggests SALSA high activity binding peptides’ (HABPs) possible role in hepatic cell invasion and merozoite invasion of erythrocytes.
ISSN:0264-410X
1873-2518
DOI:10.1016/j.vaccine.2003.09.029