Determining sources of diversity in cultivated and wild Lablab purpureus related to provenance of germplasm by using amplified fragment length polymorphism

To improve understanding of diversity of Lablab purpureus and establish relationships among 103 germplasm accessions collected from diverse geographic origins, amplified fragment length polymorphism markers were used. Four primer sets selected out of 16 produced 289 clear, repeatable polymorphisms....

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Bibliographic Details
Published in:Genetic resources and crop evolution 2005-09, Vol.52 (6), p.683-695
Main Authors: Maass, B.L, Jamnadass, R.H, Hanson, J, Pengelly, B.C
Format: Article
Language:English
Subjects:
Amplification
Amplified fragment length polymorphism
cultivars
Domestication
Genetic diversity
genetic markers
genetic variation
Germplasm
Lablab
Lablab purpureus
phylogeny
phytogeography
plant genetic resources
Polymorphism
Provenance
Taxonomy
wild plants
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Summary:To improve understanding of diversity of Lablab purpureus and establish relationships among 103 germplasm accessions collected from diverse geographic origins, amplified fragment length polymorphism markers were used. Four primer sets selected out of 16 produced 289 clear, repeatable polymorphisms. UPGMA analysis of similarity data clustered the accessions according to their subspecific taxonomic organization, i.e., subsp. purpureus and subsp. uncinatus, as well as to cultivated and wild forms. The well-represented landraces from Africa and Asia, belonging predominantly to subsp. purpureus, displayed moderate genetic diversity. Wild forms from Africa showed far greater levels of diversity that would justify taxonomic re-assessment of the wild subsp. uncinatus. The molecular analysis identified forms that were collected in the wild in India but were genetically placed intermediate between wild and cultivated forms. As these plant types did not exist among the African accessions, it is suggested that they might represent escapes from early attempts of domestication. These results support the suggested pathway of domestication and distribution of L. purpureus from Africa to Asia. Additional members to a previously published core collection of the species are proposed.
ISSN:0925-9864
1573-5109
DOI:10.1007/s10722-003-6019-3