A novel screening method for isolating exopolysaccharide-deficient mutants

A screening method based on differential staining of the wild type and exopolysaccharide-deficient mutants of Rhizobium (Sinorhizobium) meliloti by the lipophilic dye Sudan Black B is described. Mutants defective in the production of either succinoglycan or EPS II (galactoglucan) were isolated by us...

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Bibliographic Details
Published in:Applied and Environmental Microbiology 1998-11, Vol.64 (11), p.4600-4602
Main Authors: Liu, M. (Massachusetts Institute of Technology, Cambridge, MA.), Gonzalez, J.E, Willis, L.B, Walker, G.C
Format: Article
Language:English
Subjects:
Azo Compounds
Bacteria
Bacteriological Techniques
Biological and medical sciences
Biotechnology
Coloring Agents
Fundamental and applied biological sciences. Psychology
Genetic Techniques
Medical screening
Methods. Procedures. Technologies
Microbiology
MUTANT
MUTANTES
MUTANTS
Mutation
Naphthalenes
Others
Physiology and Biotechnology
POLISACARIDOS
POLYHOLOSIDE
POLYSACCHARIDES
Polysaccharides, Bacterial - biosynthesis
Polysaccharides, Bacterial - genetics
RHIZOBIUM
Rhizobium meliloti
Sinorhizobium meliloti - cytology
Sinorhizobium meliloti - genetics
Sinorhizobium meliloti - growth & development
Sinorhizobium meliloti - isolation & purification
Various methods and equipments
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Description
Summary:A screening method based on differential staining of the wild type and exopolysaccharide-deficient mutants of Rhizobium (Sinorhizobium) meliloti by the lipophilic dye Sudan Black B is described. Mutants defective in the production of either succinoglycan or EPS II (galactoglucan) were isolated by using this method, which might also prove useful for isolating exopolysaccharide-defective derivatives of other bacteria
ISSN:0099-2240
1098-5336
DOI:10.1128/aem.64.11.4600-4602.1998