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A novel screening method for isolating exopolysaccharide-deficient mutants
A screening method based on differential staining of the wild type and exopolysaccharide-deficient mutants of Rhizobium (Sinorhizobium) meliloti by the lipophilic dye Sudan Black B is described. Mutants defective in the production of either succinoglycan or EPS II (galactoglucan) were isolated by us...
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| Published in: | Applied and Environmental Microbiology 1998-11, Vol.64 (11), p.4600-4602 |
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| Main Authors: | , , , |
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| Language: | English |
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| cited_by | cdi_FETCH-LOGICAL-c550t-1eaa28e35d1c6ae05c235f97010771555700ccc18ae4e8f541957ef2f478f6e63 |
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| cites | cdi_FETCH-LOGICAL-c550t-1eaa28e35d1c6ae05c235f97010771555700ccc18ae4e8f541957ef2f478f6e63 |
| container_end_page | 4602 |
| container_issue | 11 |
| container_start_page | 4600 |
| container_title | Applied and Environmental Microbiology |
| container_volume | 64 |
| creator | Liu, M. (Massachusetts Institute of Technology, Cambridge, MA.) Gonzalez, J.E Willis, L.B Walker, G.C |
| description | A screening method based on differential staining of the wild type and exopolysaccharide-deficient mutants of Rhizobium (Sinorhizobium) meliloti by the lipophilic dye Sudan Black B is described. Mutants defective in the production of either succinoglycan or EPS II (galactoglucan) were isolated by using this method, which might also prove useful for isolating exopolysaccharide-defective derivatives of other bacteria |
| doi_str_mv | 10.1128/aem.64.11.4600-4602.1998 |
| format | article |
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(Massachusetts Institute of Technology, Cambridge, MA.) ; Gonzalez, J.E ; Willis, L.B ; Walker, G.C</creator><creatorcontrib>Liu, M. (Massachusetts Institute of Technology, Cambridge, MA.) ; Gonzalez, J.E ; Willis, L.B ; Walker, G.C</creatorcontrib><description>A screening method based on differential staining of the wild type and exopolysaccharide-deficient mutants of Rhizobium (Sinorhizobium) meliloti by the lipophilic dye Sudan Black B is described. Mutants defective in the production of either succinoglycan or EPS II (galactoglucan) were isolated by using this method, which might also prove useful for isolating exopolysaccharide-defective derivatives of other bacteria</description><identifier>ISSN: 0099-2240</identifier><identifier>EISSN: 1098-5336</identifier><identifier>DOI: 10.1128/aem.64.11.4600-4602.1998</identifier><identifier>PMID: 9797329</identifier><identifier>CODEN: AEMIDF</identifier><language>eng</language><publisher>Washington, DC: American Society for Microbiology</publisher><subject>Azo Compounds ; Bacteria ; Bacteriological Techniques ; Biological and medical sciences ; Biotechnology ; Coloring Agents ; Fundamental and applied biological sciences. Psychology ; Genetic Techniques ; Medical screening ; Methods. Procedures. Technologies ; Microbiology ; MUTANT ; MUTANTES ; MUTANTS ; Mutation ; Naphthalenes ; Others ; Physiology and Biotechnology ; POLISACARIDOS ; POLYHOLOSIDE ; POLYSACCHARIDES ; Polysaccharides, Bacterial - biosynthesis ; Polysaccharides, Bacterial - genetics ; RHIZOBIUM ; Rhizobium meliloti ; Sinorhizobium meliloti - cytology ; Sinorhizobium meliloti - genetics ; Sinorhizobium meliloti - growth & development ; Sinorhizobium meliloti - isolation & purification ; Various methods and equipments</subject><ispartof>Applied and Environmental Microbiology, 1998-11, Vol.64 (11), p.4600-4602</ispartof><rights>1999 INIST-CNRS</rights><rights>Copyright American Society for Microbiology Nov 1998</rights><rights>Copyright © 1998, American Society for Microbiology 1998</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c550t-1eaa28e35d1c6ae05c235f97010771555700ccc18ae4e8f541957ef2f478f6e63</citedby><cites>FETCH-LOGICAL-c550t-1eaa28e35d1c6ae05c235f97010771555700ccc18ae4e8f541957ef2f478f6e63</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC106691/pdf/$$EPDF$$P50$$Gpubmedcentral$$H</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC106691/$$EHTML$$P50$$Gpubmedcentral$$H</linktohtml><link.rule.ids>230,314,727,780,784,885,3188,3189,27924,27925,53791,53793</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=1722387$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/9797329$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Liu, M. (Massachusetts Institute of Technology, Cambridge, MA.)</creatorcontrib><creatorcontrib>Gonzalez, J.E</creatorcontrib><creatorcontrib>Willis, L.B</creatorcontrib><creatorcontrib>Walker, G.C</creatorcontrib><title>A novel screening method for isolating exopolysaccharide-deficient mutants</title><title>Applied and Environmental Microbiology</title><addtitle>Appl Environ Microbiol</addtitle><description>A screening method based on differential staining of the wild type and exopolysaccharide-deficient mutants of Rhizobium (Sinorhizobium) meliloti by the lipophilic dye Sudan Black B is described. Mutants defective in the production of either succinoglycan or EPS II (galactoglucan) were isolated by using this method, which might also prove useful for isolating exopolysaccharide-defective derivatives of other bacteria</description><subject>Azo Compounds</subject><subject>Bacteria</subject><subject>Bacteriological Techniques</subject><subject>Biological and medical sciences</subject><subject>Biotechnology</subject><subject>Coloring Agents</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Genetic Techniques</subject><subject>Medical screening</subject><subject>Methods. Procedures. Technologies</subject><subject>Microbiology</subject><subject>MUTANT</subject><subject>MUTANTES</subject><subject>MUTANTS</subject><subject>Mutation</subject><subject>Naphthalenes</subject><subject>Others</subject><subject>Physiology and Biotechnology</subject><subject>POLISACARIDOS</subject><subject>POLYHOLOSIDE</subject><subject>POLYSACCHARIDES</subject><subject>Polysaccharides, Bacterial - biosynthesis</subject><subject>Polysaccharides, Bacterial - genetics</subject><subject>RHIZOBIUM</subject><subject>Rhizobium meliloti</subject><subject>Sinorhizobium meliloti - cytology</subject><subject>Sinorhizobium meliloti - genetics</subject><subject>Sinorhizobium meliloti - growth & development</subject><subject>Sinorhizobium meliloti - isolation & purification</subject><subject>Various methods and equipments</subject><issn>0099-2240</issn><issn>1098-5336</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1998</creationdate><recordtype>article</recordtype><recordid>eNqFUsFu1DAQtRCobAufgBQhxC3L2I7t-MBhVRUKKuIAPVvGGW9cJfFiZwv9exx11S1cuIxHnjdvnueZkIrCmlLWvrM4rmVT8nUjAeoS2Jpq3T4hKwq6rQXn8ilZAWhdM9bAc3Ka8w0ANCDbE3KilVac6RX5vKmmeItDlV1CnMK0rUac-9hVPqYq5DjYebnE33EXh7tsnettCh3WHfrgAk5zNe5nO835BXnm7ZDx5eE8I9cfLr6fX9ZXXz9-Ot9c1U4ImGuK1rIWueiokxZBOMaF1wooKEWFEArAOUdbiw22XjRUC4We-Ua1XqLkZ-T9Pe9u_2PEzhUJyQ5ml8Jo052JNpi_K1PozTbeGgpSalr63x76U_y5xzybMWSHw2AnjPtsynwGgsF_gVQxURbKC_D1P8CbuE9TWYIpTLphnC9s7T3IpZhzQv-gmIJZTDWbiy9GNiU3i6lLYGYxtbS-evzih8aDi6X-5lC32dnBJzu5kI_8ijHeqqPMPmz7XyGhsXk05Ss9mnqc5W00dpsKz_W3okIDSEYl_wMl7b9m</recordid><startdate>19981101</startdate><enddate>19981101</enddate><creator>Liu, M. (Massachusetts Institute of Technology, Cambridge, MA.)</creator><creator>Gonzalez, J.E</creator><creator>Willis, L.B</creator><creator>Walker, G.C</creator><general>American Society for Microbiology</general><scope>FBQ</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QL</scope><scope>7QO</scope><scope>7SN</scope><scope>7SS</scope><scope>7ST</scope><scope>7T7</scope><scope>7TM</scope><scope>7U9</scope><scope>8FD</scope><scope>C1K</scope><scope>FR3</scope><scope>H94</scope><scope>M7N</scope><scope>P64</scope><scope>RC3</scope><scope>SOI</scope><scope>7X8</scope><scope>5PM</scope></search><sort><creationdate>19981101</creationdate><title>A novel screening method for isolating exopolysaccharide-deficient mutants</title><author>Liu, M. (Massachusetts Institute of Technology, Cambridge, MA.) ; Gonzalez, J.E ; Willis, L.B ; Walker, G.C</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c550t-1eaa28e35d1c6ae05c235f97010771555700ccc18ae4e8f541957ef2f478f6e63</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1998</creationdate><topic>Azo Compounds</topic><topic>Bacteria</topic><topic>Bacteriological Techniques</topic><topic>Biological and medical sciences</topic><topic>Biotechnology</topic><topic>Coloring Agents</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Genetic Techniques</topic><topic>Medical screening</topic><topic>Methods. Procedures. Technologies</topic><topic>Microbiology</topic><topic>MUTANT</topic><topic>MUTANTES</topic><topic>MUTANTS</topic><topic>Mutation</topic><topic>Naphthalenes</topic><topic>Others</topic><topic>Physiology and Biotechnology</topic><topic>POLISACARIDOS</topic><topic>POLYHOLOSIDE</topic><topic>POLYSACCHARIDES</topic><topic>Polysaccharides, Bacterial - biosynthesis</topic><topic>Polysaccharides, Bacterial - genetics</topic><topic>RHIZOBIUM</topic><topic>Rhizobium meliloti</topic><topic>Sinorhizobium meliloti - cytology</topic><topic>Sinorhizobium meliloti - genetics</topic><topic>Sinorhizobium meliloti - growth & development</topic><topic>Sinorhizobium meliloti - isolation & purification</topic><topic>Various methods and equipments</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Liu, M. 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(Massachusetts Institute of Technology, Cambridge, MA.)</au><au>Gonzalez, J.E</au><au>Willis, L.B</au><au>Walker, G.C</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>A novel screening method for isolating exopolysaccharide-deficient mutants</atitle><jtitle>Applied and Environmental Microbiology</jtitle><addtitle>Appl Environ Microbiol</addtitle><date>1998-11-01</date><risdate>1998</risdate><volume>64</volume><issue>11</issue><spage>4600</spage><epage>4602</epage><pages>4600-4602</pages><issn>0099-2240</issn><eissn>1098-5336</eissn><coden>AEMIDF</coden><abstract>A screening method based on differential staining of the wild type and exopolysaccharide-deficient mutants of Rhizobium (Sinorhizobium) meliloti by the lipophilic dye Sudan Black B is described. Mutants defective in the production of either succinoglycan or EPS II (galactoglucan) were isolated by using this method, which might also prove useful for isolating exopolysaccharide-defective derivatives of other bacteria</abstract><cop>Washington, DC</cop><pub>American Society for Microbiology</pub><pmid>9797329</pmid><doi>10.1128/aem.64.11.4600-4602.1998</doi><tpages>3</tpages><oa>free_for_read</oa></addata></record> |
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| ispartof | Applied and Environmental Microbiology, 1998-11, Vol.64 (11), p.4600-4602 |
| issn | 0099-2240 1098-5336 |
| language | eng |
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| source | PubMed Central(OpenAccess); American Society for Microbiology Journals |
| subjects | Azo Compounds Bacteria Bacteriological Techniques Biological and medical sciences Biotechnology Coloring Agents Fundamental and applied biological sciences. Psychology Genetic Techniques Medical screening Methods. Procedures. Technologies Microbiology MUTANT MUTANTES MUTANTS Mutation Naphthalenes Others Physiology and Biotechnology POLISACARIDOS POLYHOLOSIDE POLYSACCHARIDES Polysaccharides, Bacterial - biosynthesis Polysaccharides, Bacterial - genetics RHIZOBIUM Rhizobium meliloti Sinorhizobium meliloti - cytology Sinorhizobium meliloti - genetics Sinorhizobium meliloti - growth & development Sinorhizobium meliloti - isolation & purification Various methods and equipments |
| title | A novel screening method for isolating exopolysaccharide-deficient mutants |
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