Activation of the insulin‐like growth factor‐1 receptor promotes the survival of human keratinocytes following ultraviolet B irradiation

• Published in: International journal of cancer 1999-01, Vol.80 (3), p.431-438
• Main Authors: Kuhn, Christine, Hurwitz, Steven A., Kumar, Manish G., Cotton, Jenny, Spandau, Dan F.
• Format: Article
• Language: English
• Subjects: Apoptosis - genetics; Biological and medical sciences; Calcium-Calmodulin-Dependent Protein Kinases - metabolism; Cell Division; Cell Survival - drug effects; Cell Survival - radiation effects; Cells, Cultured; Culture Media; Dermatology; DNA - radiation effects; DNA Fragmentation; Enzyme Activation; Humans; Insulin - pharmacology; Insulin-Like Growth Factor I - physiology; Keratinocytes - drug effects; Keratinocytes - metabolism; Keratinocytes - radiation effects; Medical sciences; Phosphatidylinositol 3-Kinases - metabolism; Radiation Dosage; Receptor, IGF Type 1 - metabolism; Tumors of the skin and soft tissue. Premalignant lesions; Ultraviolet Rays
• ISSN: 0020-7136; 1097-0215
• DOI: 10.1002/(SICI)1097-0215(19990129)80:3<431::AID-IJC16>3.0.CO;2-5

The ultraviolet B (UVB) component of sunlight causes non‐melanoma skin cancers due to the damage it inflicts on genomic DNA. The response of epidermal keratinocytes to sunlight depends on the dose of UVB received and the severity of the damage to the DNA. Mild DNA damage typically induces DNA‐repair pathways and cell survival, while severe DNA damage provokes apoptosis. Primary human keratinocytes grown in serum‐free media respond in a similar manner to UVB irradiation. However, we observed that keratinocytes are exquisitely more susceptible to UVB‐induced apoptosis if the growth medium is depleted of exogenous growth factors. Therefore, an exogenous growth factor could provide protection from UVB‐induced apoptosis. We found that the only growth factor that provided protection from UVB‐induced apoptosis was insulin and that the protective effect elicited by insulin was not due to binding the insulin receptor but, rather, to activation of the insulin‐like growth factor‐1 (IGF‐1) receptor. Additionally, activation of the IGF‐1 receptor in combination with UVB irradiation induced keratinocytes to become post‐mitotic. This survival function of the IGF‐1 receptor in response to UVB irradiation was influenced by activation of phosphatidylinositol‐3 kinase and MAP kinase. Prior to UVB irradiation, insulin or IGF‐1 had little to no effect on cell growth or viability. Therefore, activation of the IGF‐1 receptor in conjunction with UVB irradiation promotes keratinocyte survival at the expense of cell proliferation. Int. J. Cancer 80:431–438, 1999. © 1999 Wiley‐Liss, Inc.