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Induction of Arylhydrocarbon Receptor Expression in Embryoblast Cells of Rabbit Preimplantation Blastocysts upon Degeneration of Rauber's Polar Trophoblast

The arylhydrocarbon receptor (AhR) is a ligand-activated transcription factor and mediates carcinogenic, teratogenic, and toxic effects of xenobiotics such as dioxin and coplanar polychlorinated biphenyls. The AhR nuclear translocator (ARNT) is involved in AhR signal transduction. We have analyzed t...

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Published in:Toxicology and applied pharmacology 1999-06, Vol.157 (2), p.125-133
Main Authors: Tscheudschilsuren, G., Küchenhoff, A., Klonisch, T., Tetens, F., Fischer, B.
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cited_by cdi_FETCH-LOGICAL-c466t-8769a16182ac1500d7f7d1ce1818b29f4774bee66efc907eac892d03bac4e4863
cites cdi_FETCH-LOGICAL-c466t-8769a16182ac1500d7f7d1ce1818b29f4774bee66efc907eac892d03bac4e4863
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container_issue 2
container_start_page 125
container_title Toxicology and applied pharmacology
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creator Tscheudschilsuren, G.
Küchenhoff, A.
Klonisch, T.
Tetens, F.
Fischer, B.
description The arylhydrocarbon receptor (AhR) is a ligand-activated transcription factor and mediates carcinogenic, teratogenic, and toxic effects of xenobiotics such as dioxin and coplanar polychlorinated biphenyls. The AhR nuclear translocator (ARNT) is involved in AhR signal transduction. We have analyzed the expression of AhR and ARNT mRNA and AhR protein in Day 3 pc (postcoitum) rabbit morulae and Days 4 and 6 pc blastocysts using RT–PCR, nested PCR, whole mount in situ hybridization, and whole mount immunohistochemistry with subsequent confocal laser scanning analysis. AhR and ARNT transcripts were detected in all stages investigated, indicating coexpression of both transcription factors. AhR protein was localized in the cytoplasm. It was detected in Day 3 pc morulae and in blastocysts. In Day 4 pc blastocysts, only trophoblast cells but not embryoblast cells were immunopositive. However, at Day 6 pc, the embryoblast cells also expressed AhR protein and this expression was correlated with the degeneration of Rauber's trophoblast layer.
doi_str_mv 10.1006/taap.1999.8667
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ispartof Toxicology and applied pharmacology, 1999-06, Vol.157 (2), p.125-133
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1096-0333
language eng
recordid cdi_proquest_miscellaneous_17252976
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subjects AhR nuclear translocator (ARNT)
Animals
Aryl Hydrocarbon Receptor Nuclear Translocator
arylhydrocarbon receptor (AhR)
Biological and medical sciences
Blastocyst - chemistry
Blastocyst - cytology
Blastocyst - metabolism
Blotting, Western
Cytoplasm - chemistry
Cytoplasm - metabolism
DNA-Binding Proteins
Embryonic Development
Environmental pollutants toxicology
Female
Gene Expression Regulation, Developmental
General aspects
Immunohistochemistry
In Situ Hybridization
Medical sciences
Pregnancy
preimplantation embryos
rabbit
Rabbits
Rauber's polar trophoblast
Receptors, Aryl Hydrocarbon - analysis
Receptors, Aryl Hydrocarbon - genetics
Receptors, Aryl Hydrocarbon - metabolism
Reverse Transcriptase Polymerase Chain Reaction
RNA, Messenger - metabolism
Sequence Homology, Nucleic Acid
Toxicology
Transcription Factors - genetics
Trophoblasts - cytology
Trophoblasts - metabolism
Trophoblasts - physiology
title Induction of Arylhydrocarbon Receptor Expression in Embryoblast Cells of Rabbit Preimplantation Blastocysts upon Degeneration of Rauber's Polar Trophoblast
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