Protection by Group II Phospholipase A sub(2) Against Staphylococcus aureus

Group II phospholipase A sub(2) (PLA sub(2)) is an enzyme that has marked antibacterial properties in vitro. To define the role of group II PLA sub(2) in the defense against Staphylococcus aureus, we studied host responses in transgenic mice expressing human group II PLA sub(2) and group II PLA sub(...

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Bibliographic Details
Published in:The Journal of immunology (1950) 1999-06, Vol.162 (12), p.7402-7408
Main Authors: Laine, VJO, Grass, D S, Nevalainen, T J
Format: Article
Language:English
Subjects:
Staphylococcus aureus
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Summary:Group II phospholipase A sub(2) (PLA sub(2)) is an enzyme that has marked antibacterial properties in vitro. To define the role of group II PLA sub(2) in the defense against Staphylococcus aureus, we studied host responses in transgenic mice expressing human group II PLA sub(2) and group II PLA sub(2)-deficient C57BL/6J mice in experimental S. aureus infection. After the administration of S. aureus, the transgenic mice showed increased expression of group II PLA sub(2) mRNA in the liver and increased concentration of group II PLA sub(2) in serum, whereas the PLA sub(2)-deficient mice completely lacked the PLA sub(2) response. Expression of human group II PLA sub(2) resulted in reduced mortality and improved the resistence of the mice by killing the bacteria as indicated by low numbers of live bacteria in their tissues. Human group II PLA sub(2) was responsible for the bactericidal activity of transgenic mouse serum. These results suggest a possible role for group II PLA sub(2) in the innate immunity against S. aureus infection.
ISSN:0022-1767