Neuroprotective activity of the potent and selective mGlu1a metabotropic glutamate receptor antagonist, (+)-2-methyl-4 carboxyphenylglycine (LY367385): comparison with LY357366, a broader spectrum antagonist with equal affinity for mGlu1a and mGlu5 receptors

• Published in: Neuropharmacology 1999-02, Vol.38 (2), p.199-207
• Main Authors: Bruno, V, Battaglia, G, Kingston, A, O’Neill, M.J, Catania, M.V, Di Grezia, R, Nicoletti, F
• Format: Article
• Language: English
• Subjects: Animals; Astrocytes - cytology; Astrocytes - drug effects; Astrocytes - physiology; Benzoates; Biological and medical sciences; Caudate Nucleus - cytology; Caudate Nucleus - drug effects; Caudate Nucleus - pathology; Cells, Cultured; Cerebral Cortex - cytology; Cerebral Cortex - physiology; Excitatory Amino Acid Antagonists - pharmacology; Fetus; Gerbillinae; Global ischemia; Glycine - analogs & derivatives; Glycine - pharmacology; Group-I metabotropic glutamate receptors; Ischemic Attack, Transient - pathology; LY367366; LY367385; Male; Medical sciences; Mice; N-methyl- d-aspartate toxicity; N-Methylaspartate - toxicity; Necrosis; Neurons - cytology; Neurons - drug effects; Neurons - pathology; Neuropharmacology; Neuroprotection; Neuroprotective agent; Neuroprotective Agents - pharmacology; Pharmacology. Drug treatments; Rats; Rats, Sprague-Dawley; Receptor, Metabotropic Glutamate 5; Receptors, Metabotropic Glutamate - antagonists & inhibitors; Receptors, Metabotropic Glutamate - physiology; Thiophenes - pharmacology
• ISSN: 0028-3908; 1873-7064
• DOI: 10.1016/S0028-3908(98)00159-2

(+)-2-Methyl-4-carboxyphenylglycine (LY367385), a potent and selective antagonist of mGlu1a metabotropic glutamate receptors, was neuroprotective in the following in vitro and in vivo models of excitotoxic death: (i) mixed cultures of murine cortical cells transiently exposed to N-methyl- d-aspartate (NMDA); (ii) rats monolaterally infused with NMDA into the caudate nucleus; and (iii) gerbils subjected to transient global ischemia. We have compared the activity of LY367385 with that of the novel compound (±)- α-thioxantylmethyl-4-carboxyphenylglycine (LY367366), which antagonizes both mGlu1a and -5 receptors at low micromolar concentrations, but also recruits other subtypes at higher concentrations. Although LY367366 was neuroprotective, it was in general less efficacious than LY357385, suggesting that inhibition of mGlu1 receptors is sufficient to confer significant neuroprotection. We conclude that endogenous activation of mGlu1a receptors (or perhaps other mGlu1 receptor splice variants) contributes to the development of neuronal degeneration of excitotoxic origin.