Marked Expression of Glutathione S-Transferase A4-4 Detoxifying 4-Hydroxy-2(E)-nonenal in the Skin of Rats Irradiated by Ultraviolet B-Band Light (UVB)

Enzyme, Western blot, and immunohistochemical analyses indicated that rat skin cytosol contained no detectable level of the homodimeric, alpha-class glutathione S-transferase (rGST) A4-4 which catalyzes the GSH conjugation of the toxic product, 4-hydroxy-2(E)-nonenal (HNE), nonenzymatically formed f...

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Bibliographic Details
Published in:Biochemical and biophysical research communications 1999-07, Vol.260 (3), p.740-746
Main Authors: Hiratsuka, Akira, Saito, Hiroshi, Hirose, Kenichiro, Watabe, Tadashi
Format: Article
Language:English
Subjects:
Aldehydes - metabolism
Aldehydes - toxicity
Animals
Blotting, Western
Chromatography, High Pressure Liquid
Cytosol - enzymology
Cytosol - metabolism
Cytosol - radiation effects
Gene Expression Regulation, Enzymologic - radiation effects
Glutathione - metabolism
Glutathione Transferase - chemistry
Glutathione Transferase - isolation & purification
Glutathione Transferase - metabolism
Immunohistochemistry
Inactivation, Metabolic
Isoenzymes - metabolism
Liver - enzymology
Male
Rats
Rats, Sprague-Dawley
Sequence Analysis
Skin - cytology
Skin - enzymology
Skin - metabolism
Skin - radiation effects
Ultraviolet Rays
Up-Regulation - radiation effects
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Summary:Enzyme, Western blot, and immunohistochemical analyses indicated that rat skin cytosol contained no detectable level of the homodimeric, alpha-class glutathione S-transferase (rGST) A4-4 which catalyzes the GSH conjugation of the toxic product, 4-hydroxy-2(E)-nonenal (HNE), nonenzymatically formed from n-6 polyunsaturated fatty acid residues of lipids by lipid peroxidation. Rats irradiated by single doses (4000–24,000 mJ/cm2) of ultraviolet B-band light (UVB, 200 mJ/cm2/min) markedly expressed rGSTA4-4 in the skin at a level one-fifth that of the liver in apparent specific activity toward HNE at a single dose of 24,000 mJ/cm2. Skin rGSTA4-4 was isolated, purified to homogeneity, and identified with hepatic rGSTA4-4 by reverse-phase partition HPLC and by amino acid sequence analysis of its CNBr fission peptides. Immunohistochemistry with polyclonal antibody raised against rGSTA4-4 demonstrated the selective expression of rGSTA4-4 in epidermis and sebaceous glands localized in dermis after UVB irradiation.
ISSN:0006-291X
1090-2104
DOI:10.1006/bbrc.1999.0971