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Crystallographic and Functional Studies of Very Short Patch Repair Endonuclease

Vsr endonuclease plays a crucial role in the repair of TG mismatched base pairs, which are generated by the spontaneous degradation of methylated cytidines; Vsr recognizes the mismatched base pair and cleaves the phosphate backbone 5′ to the thymidine. We have determined the crystal structure of a t...

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Published in:Molecular cell 1999-05, Vol.3 (5), p.621-628
Main Authors: Tsutakawa, Susan E, Muto, Takanori, Kawate, Tomohiko, Jingami, Hisato, Kunishima, Naoki, Ariyoshi, Mariko, Kohda, Daisuke, Nakagawa, Masako, Morikawa, Kosuke
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cited_by cdi_FETCH-LOGICAL-c505t-25857c2baafa96132dc6a7fa721af67d99db75d0d24757b920d8177e00b7ed8c3
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container_title Molecular cell
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creator Tsutakawa, Susan E
Muto, Takanori
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Jingami, Hisato
Kunishima, Naoki
Ariyoshi, Mariko
Kohda, Daisuke
Nakagawa, Masako
Morikawa, Kosuke
description Vsr endonuclease plays a crucial role in the repair of TG mismatched base pairs, which are generated by the spontaneous degradation of methylated cytidines; Vsr recognizes the mismatched base pair and cleaves the phosphate backbone 5′ to the thymidine. We have determined the crystal structure of a truncated form of this endonuclease at 1.8 Å resolution. The protein contains one structural zinc-binding module. Unexpectedly, its overall topology resembles members of the type II restriction endonuclease family. Subsequent mutational and biochemical analyses showed that certain elements in the catalytic site are also conserved. However, the identification of a critical histidine and evidence of an active site metal-binding coordination that is novel to endonucleases indicate a distinct catalytic mechanism.
doi_str_mv 10.1016/S1097-2765(00)80355-X
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subjects Alanine
Base Pair Mismatch
Catalytic Domain
Conserved Sequence
Crystallography
Deoxyribonucleases, Type II Site-Specific - chemistry
Deoxyribonucleases, Type II Site-Specific - genetics
Deoxyribonucleases, Type II Site-Specific - metabolism
DNA Repair
Endodeoxyribonucleases - chemistry
Endodeoxyribonucleases - genetics
Endodeoxyribonucleases - metabolism
Manganese - metabolism
Molecular Sequence Data
Mutagenesis
Protein Structure, Secondary
Protein Structure, Tertiary
Sequence Homology, Amino Acid
Static Electricity
title Crystallographic and Functional Studies of Very Short Patch Repair Endonuclease
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