Splicing-independent processing of plant box C/D and box H/ACA small nucleolar RNAs

Small nucleolar RNAs (snoRNAs) are involved in various aspects of ribosome biogenesis and rRNA maturation. Plants have a unique organisation of snoRNA genes where multiple, different genes are tightly clustered at a number of different loci. The maize gene clusters studied here include genes from bo...

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Bibliographic Details
Published in:Plant molecular biology 1999-04, Vol.39 (6), p.1091-1100
Main Authors: Leader, D.J, Clark, G.P, Watters, J, Beven, A.F, Shaw, P.J, Brown, J.W.S
Format: Article
Language:English
Subjects:
Base Sequence
Biosynthesis
cell nucleolus
Endonucleases - metabolism
enzyme activity
Gene loci
genes
Genes - genetics
Genes, Plant - genetics
Genetic Vectors
hydrolysis
intronic transcripts
Introns - genetics
Models, Genetic
Nicotiana - genetics
non-intronic transcripts
Plant biology
Plants, Toxic
polycistronic transcripts
Promoter Regions, Genetic - genetics
Protoplasts
Regulatory Sequences, Nucleic Acid - genetics
ribonucleases
Ribonucleic acid
RNA
RNA Processing, Post-Transcriptional - genetics
RNA Splicing
RNA, Plant - analysis
RNA, Plant - genetics
RNA, Plant - metabolism
RNA, Small Nuclear - analysis
RNA, Small Nuclear - classification
RNA, Small Nuclear - genetics
RNA, Small Nuclear - metabolism
Transfection
Yeasts
Zea mays
Zea mays - enzymology
Zea mays - genetics
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Summary:Small nucleolar RNAs (snoRNAs) are involved in various aspects of ribosome biogenesis and rRNA maturation. Plants have a unique organisation of snoRNA genes where multiple, different genes are tightly clustered at a number of different loci. The maize gene clusters studied here include genes from both of the two major classes of snoRNAS (box C/D and box H/ACA) and are transcribed as a polycistronic pre-snoRNA transcript from an upstream promoter. In contrast to vertebrate and yeast intron-encoded snoRNAs, which are processed from debranched introns by exonuclease activity, the particular organisation of plant snoRNA genes suggests a different mode of expression and processing. Here we show that single and multiple plant snoRNAs can be processed from both non-intronic and intronic transcripts such that processing is splicing-independent and requires endonucleolytic activity. Processing of these different snoRNAs from the same polycistronic transcript suggests that the processing machineries needed by each class are not spatially separated in the nucleolus/nucleus.
ISSN:0167-4412
1573-5028
DOI:10.1023/a:1006157022319