Long terminal repeat sequences of equine infectious anaemia virus are a major determinant of cell tropism

SL Payne, K La Celle, XF Pei, XM Qi, H Shao, WK Steagall, S Perry and F Fuller Biology Department, The University of Texas at Arlington, 76019-0498, USA. spayne@utarlg.uta.edu The Wyoming strain of equine infectious anaemia virus (EIAV) is a highly virulent field strain that replicates to high titre...

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Published in:Journal of general virology 1999-03, Vol.80 (3), p.755-759
Main Authors: Payne, SL, La Celle, K, Pei, XF, Qi, XM, Shao, H, Steagall, WK, Perry, S, Fuller, F
Format: Article
Language:English
Subjects:
Animals
Base Sequence
Cell Line
Consensus Sequence - genetics
Equine infectious anemia virus
Fibroblasts - virology
Gene Expression Regulation, Viral
Gene Products, tat - genetics
Gene Products, tat - metabolism
Genes, Reporter
Genetic Variation
Horses - virology
Infectious Anemia Virus, Equine - classification
Infectious Anemia Virus, Equine - genetics
Infectious Anemia Virus, Equine - growth & development
Infectious Anemia Virus, Equine - pathogenicity
Macrophages - virology
Molecular Sequence Data
Mutagenesis, Site-Directed
Promoter Regions, Genetic - genetics
Terminal Repeat Sequences - genetics
Transcriptional Activation
Transfection
Wyoming
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Summary:SL Payne, K La Celle, XF Pei, XM Qi, H Shao, WK Steagall, S Perry and F Fuller Biology Department, The University of Texas at Arlington, 76019-0498, USA. spayne@utarlg.uta.edu The Wyoming strain of equine infectious anaemia virus (EIAV) is a highly virulent field strain that replicates to high titre in vitro only in primary equine monocyte-derived macrophages. In contrast, Wyoming-derived fibroblast-adapted EIAV strains (Malmquist virus) replicate in primary foetal equine kidney and equine dermis cells as well as in the cell lines FEA and Cf2Th. Wyoming and Malmquist viruses differ extensively both in long terminal repeat (LTR) and envelope region sequences. We have compared the promoter activities of the Wyoming LTR with those of LTRs derived from fibroblast-adapted viruses by examining their abilities to drive a luciferase reporter gene as well as by construction of infectious molecular clones differing only in LTR sequence. Our results indicate that LTR sequences are a major restriction for growth of the Wyoming strain of EIAV in fibroblasts.
ISSN:0022-1317
1465-2099
DOI:10.1099/0022-1317-80-3-755