Random and repetitive primer amplified polymorphic DNA analysis of Bacillus sphaericus

A comparative study of 15 strains representing the five homology groups of Bacillus sphaericus was performed by two PCR methods: RAPD and rep-PCR fingerprinting. The PCR analysis performed with primers corresponding to the naturally occurring repetitive sequences REP, ERIC and BOX, as well as with t...

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Bibliographic Details
Published in:Journal of applied microbiology 1999-06, Vol.86 (6), p.928-936
Main Authors: Miteva, V, Selenska-Pobell, S, Mitev, V
Format: Article
Language:English
Subjects:
Bacillus sphaericus
Bacteriological methods and techniques used in bacteriology
Bacteriology
Biological and medical sciences
chemotaxonomy
Culicidae
Fundamental and applied biological sciences. Psychology
Microbiology
pathogens
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Summary:A comparative study of 15 strains representing the five homology groups of Bacillus sphaericus was performed by two PCR methods: RAPD and rep-PCR fingerprinting. The PCR analysis performed with primers corresponding to the naturally occurring repetitive sequences REP, ERIC and BOX, as well as with three random primers, showed highly variable patterns and allowed differentiation of the strains studied. This demonstrated the high discriminative power of the methods. The cluster analysis revealed a low level of similarity between the different homology groups, and within groups I and III, which is evidence of the high genetic heterogeneity of the species B. sphaericus. Close genetic relatedness was observed for the representatives of group IIA, pathogenic to mosquitoes, which supports the idea for differentiation of this group as a separate species.
ISSN:1364-5072
1365-2672
DOI:10.1046/j.1365-2672.1999.00769.x