Monitoring gene expression profile changes in ovarian carcinomas using cDNA microarray

• Published in: Gene 1999-03, Vol.229 (1), p.101-108
• Main Authors: Wang, Kai, Gan, Lu, Jeffery, Eric, Gayle, Margit, Gown, Allen M., Skelly, Marilyn, Nelson, Peter S., Ng, WeiLap V., Schummer, Michèl, Hood, Leroy, Mulligan, John
• Format: Article
• Language: English
• Subjects: Array; Carcinogenesis; Cloning, Molecular; DNA, Complementary - genetics; Expressed Sequence Tags; Female; Gene Expression Regulation, Neoplastic - genetics; Genes, Neoplasm - genetics; GPI-Linked Proteins; Humans; Membrane Glycoproteins - genetics; Oligonucleotide Array Sequence Analysis; Ovarian cancer; Ovarian Neoplasms - genetics; Polymerase Chain Reaction; Reverse Transcriptase Polymerase Chain Reaction; RNA, Messenger - genetics; RNA-Directed DNA Polymerase - genetics; RT–PCR; Tumor marker
• ISSN: 0378-1119; 1879-0038
• DOI: 10.1016/S0378-1119(99)00035-9

The development of cancer is the result of a series of molecular changes occurring in the cell. These events lead to changes in the expression level of numerous genes that result in different phenotypic characteristics of tumors. In this report we describe the assembly and utilization of a 5766 member cDNA microarray to study the differences in gene expression between normal and neoplastic human ovarian tissues. Several genes that may have biological relevance in the process of ovarian carcinogenesis have been identified through this approach. Analyzing the results of microarray hybridizations may provides new leads for tumor diagnosis and intervention.