Frequency and haplotype association of HLA-DQB103:19 in Saudi Arabian population

Background The relatively new HLA-antigen DQB10319 allele was identified in 2006 and included as common allele in the updated common and well documented allele (CWD) list. HLA-DQB103:19 allele differs from DQB103:01allele at nucleotide position 554 (CT) in Exon 2, which results in Thr to Ile amino a...

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Published in:Human immunology 2015-10, Vol.76, p.75-75
Main Authors: Al-Otaibi, Ahmed, Shawhatti, Adel, Zahrani, Saber, Al-Attas, Rabab, Liacini, Abdelhamid
Format: Article
Language:English
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Summary:Background The relatively new HLA-antigen DQB10319 allele was identified in 2006 and included as common allele in the updated common and well documented allele (CWD) list. HLA-DQB103:19 allele differs from DQB103:01allele at nucleotide position 554 (CT) in Exon 2, which results in Thr to Ile amino acid exchange. This allele shows a strong association with DRB111:02. Rare association with DRB113:04 had been also reported. Objective To assess the frequency of DQB103:19 allele in Saudi population we reviewed all HLA- typing results of our database (4000). The samples represent patients and corresponding donors referred to the only transplant center covering the largest province in Saudi Arabia during five years period. Methods Medium resolution typing for HLA-DRB1 and -DQB1 was performed by Luminex-based SSO method. Sequencing based typing (SBT) was performed by Atria Genetics HLA SBT kits and analyzed on ABI 3130 Genetic Analyzer using ASSIGN 3.6 software (Conexio Genomics). Results HLA DQA105:05 -DQB103:19 allele was observed in 124 (3%) individuals. The data showed that the majority of these DQB103:19 samples (65%) were also positive for either, DRB111:01 or DRB111:02 and DRB302:02 alleles, suggesting that the haplotype DRB111:01/02, DRB302:02, is the commonest haplotype having this antigen. The next common haplotype was DQB103:19, DRB108:04 (15%), followed by DQB103:19, DRB107:03 (11%) followed by DQB103:19, DRB103:01 (9%). Conclusion Our data suggests that DQB103:19 is observed frequently in Saudi population and the commonest haplotype reported is in agreement with the known DRB111:01/02 association, however unusual association with DRB108:04 or 07:03 or 03:01 probably is unique to our population.
ISSN:0198-8859
DOI:10.1016/j.humimm.2015.07.107