Mechanism of Incompatible Herb Pairs, Panax ginseng and Veratrum nigrum L,： Material Basis and Metabolic Profiles of Ginsenosides in Rat Intestinal Bacteria

• Published in: Chinese journal of chemistry 2015-09, Vol.33 (9), p.1069-1076
• Main Authors: Yang, Xiudong, Li, Xue, Xu, Ying, Pi, Zifeng, Lin, Na, Liu, Zhiqiang, Song, Fengrui
• Format: Article
• Language: English
• Subjects: Bacteria; Chinese medicine; Herbs; incompatibility; Mass spectrometry; Metabolism; Panax ginseng; rat intestinal bacteria; UPLC-MS; Veratrum; Veratrum nigrum L; 不相容; 人参皂苷; 代谢特征; 大鼠; 机制; 物质基础; 肠道菌群; 藜芦
• ISSN: 1001-604X; 1614-7065
• DOI: 10.1002/cjoc.201500307

In traditional Chinese medicine theory, Panax ginseng and Veratrum nigrum L. is an important incompatible herb pair. Studies on the content variation of main components and the influences on the metabolism in rat intestinal bacteria are useful to understand the mechanism of incompatibility of this herb pairs. In this study, the content variation of ginsenosides and their metaboltic profiles in the extracts of P. ginseng and compatibility of P. ginseng with V. nigrum L. （G-V） were investigated using relative quantitative method of electrospray ionization mass spec- trometry （ESI-MS） and UPLC-MSn, respectively. The relative contents of most ginsenosides were reduced in the extract of G-V. Furthermore, ginsenosides Rbt, Rb2, Rc and Rd could be metabolized to Rd, F2 and C-K in rat in- testinal bacteria. The metabolic speeds ofRbl, Rb2 and Re in the G-V extracts at ratios of 10 ： 5, 10 ： 7 and 10 ： 10 and the metabolic rates of ginsenosides Rbb Rb2 and Rc to Rd, Rd to F2 in all compatibility extracts were lower than that in the P ginseng extract. In conclusion, this study illustrated the mechanism of effect-reducing by comparison of the relative contents and metabolic profiles of ginsenosides after compatibility of P ginseng and V. nigrum L.